Peptides as standards for denaturing isoelectric focusing
Peptides as standards for denaturing isoelectric focusing
复制标题
肽作为变性等电聚焦的标准
DOI：
10.1002/elps.1150160106
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发表时间：
1995
期刊：
影响因子：
2.9
通讯作者：
R. Singh
中科院分区：
文献类型：
--
作者：
N. Watts;R. Singh
描述了一组适合用作变性等电聚焦（IEF）标准的市售肽。肽N -降钙素原片段1-57 (pI 3.98)， Gln11 -淀粉样β蛋白片段1-28 (pI 5.76)，胃抑制多肽（pI 7.14），甲状旁腺激素片段1-34 （pI 8.64）和人β -内啡肽（pI 9.49）可以在8 M尿素和2% Nonidet P-40存在下聚焦到它们的等电点，然后在聚丙烯酰胺凝胶中固定和标记。肽的线性标准曲线与表面pH电极测定的斜率密切一致。在同样的条件下，一些蛋白质聚焦到与其理论等电点明显不一致的位置。讨论了这些差异的起源和对未知蛋白质的等电点变性的影响。
A set of commercially available peptides suitable for use as standards in denaturing isoelectric focusing (IEF) is described. The peptides N‐procalcitonin fragment 1–57 (pI 3.98), Gln11‐amyloid β‐protein fragment 1–28 (pI 5.76), gastric inhibitory polypeptide (pI 7.14), parathyroid hormone fragment 1–34 (pI 8.64) and human β‐endorphin (pI 9.49) can be focused to their isoelectric point in the presence of 8 M urea and 2% Nonidet P–40, and subsequently fixed and stained in polyacrylamide gels. The peptides give a linear standard curve in close agreement with a slope determined with a surface pH electrode. Under the same conditions some proteins focus to positions significantly at odds with their theoretical isoelectric point. The origins of these discrepancies and the implications for the determination of isoelectric points of unknown proteins by denaturing IEF are discussed.
通过质子磁共振和氢交换对蛋白质折叠中间体进行结构表征。
影响因子：
--
作者：
Roder,H
通讯作者：
Roder,H
胰蛋白酶抑制剂中的氢交换率与尿素的热稳定性不相关。
影响因子：
2.9
作者：
Hilton,BD;Trudeau,K;Woodward,CK
通讯作者：
Woodward,CK
影响因子：
5.6
作者：
GILSON, MK;RASHIN, A;HONIG, B
通讯作者：
HONIG, B