Eradication of bacteria in suspension and biofilms using methylene blue-loaded dynamic nanoplatforms.
Eradication of bacteria in suspension and biofilms using methylene blue-loaded dynamic nanoplatforms.
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使用载有亚甲蓝的动态纳米平台消灭悬浮液和生物膜中的细菌。
DOI：
10.1128/aac.01604-08
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发表时间：
2009
影响因子：
4.9
通讯作者：
Xi,Chuanwu
中科院分区：
文献类型：
--
作者：
Wu,Jianfeng;Xu,Hao;Tang,Wei;Kopelman,Raoul;Philbert,MartinA;Xi,Chuanwu
评价了动态纳米平台（DNP）的细菌杀灭效率。DNP的聚丙烯酰胺（PAA）水凝胶基质负载有亚甲蓝（MB），并且先前成功地应用于杀死培养中的大鼠C6胶质瘤肿瘤细胞。这一系列实验旨在确定这种纳米平台消除细菌感染的适用性。金黄色葡萄球菌、铜绿假单胞菌、大肠杆菌和不动杆菌的悬浮培养物。暴露于活化的（100650-nm激光）MB-PAA-DNP。测定了纳米颗粒质量浓度、光辐照度和能量密度以及黑暗孵育时间对每种细菌的杀灭效率。此外，证明了活化的MB-PAA-DNP抑制生物膜生长并根除和分散在玻璃和聚苯乙烯表面上预形成的预形成生物膜的能力。数据显示，活化的MB-PAA-DNP根除了所有检查的细菌物种。此外，将MB包封到PAA-DNP基质中显著降低了观察到的游离染料的暗毒性。发现MB-PAA-DNP的光杀菌功效对于革兰氏阳性细菌比对于革兰氏阴性细菌更高。此外，活化的MB-PAA-DNP可以抑制生物膜的生长，并消除几乎所有的早期生物膜，所述早期生物膜是由所有被检查的细菌形成的。
The bacterial killing efficiency of a dynamic nanoplatform (DNP) was evaluated. The polyacrylamide (PAA) hydrogel matrix of the DNP was loaded with methylene blue (MB) and was previously applied successfully to killing rat C6 glioma tumor cells in culture. This series of experiments is aimed at determining the suitability of this nanoplatform for elimination of bacterial infections. Suspended cultures ofStaphylococcus aureus,Pseudomonas aeruginosa,Escherichia coli, andAcinetobactersp. were exposed to activated (∼650-nm laser light) MB-PAA-DNPs. The killing efficiency of nanoparticle mass concentration, light irradiance and fluence, and dark incubation time was determined on each of the bacterial species. Moreover, the ability of activated MB-PAA-DNPs to inhibit biofilm growth and eradicate and disperse preformed biofilms, preformed on glass and polystyrene surfaces, was demonstrated. The data revealed that activated MB-PAA-DNPs eradicated all species of bacteria examined. Also, encapsulation of MB into the PAA-DNP matrix significantly diminished the observed dark toxicity of free dye. The photobactericidal efficacy of MB-PAA-DNP was found to be higher for gram-positive bacteria than for gram-negative bacteria. In addition, activated MB-PAA-DNP can inhibit biofilm growth and eradicate almost all of the early-age biofilms that are formed by all of the bacteria examined.