The lipidome and proteome of oil bodies from Helianthus annuus (common sunflower).
The lipidome and proteome of oil bodies from Helianthus annuus (common sunflower).
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DOI：
10.1007/s12154-012-0090-1
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发表时间：
2013-04
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影响因子：
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通讯作者：
Gray, David A
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作者：
Furse, Samuel;Liddell, Susan;Ortori, Catharine A;Williams, Huw;Neylon, D Cameron;Scott, David J;Barrett, David A;Gray, David A
在本文中，我们报告的分子概况，脂质组和蛋白质组，植物细胞器被称为油体（OB）。OB的显著之处在于它能够发挥其生物学作用（储存甘油三酯），同时抵抗由干燥（脱水）和发芽（再水化）期间的变化引起的物理应力。使用31 P/1H核磁共振（NMR），高分辨率质谱和标称质量串联质谱的脂质组，和凝胶电泳色谱串联质谱的蛋白质组的组合，确定的分子概况，赋予这种非凡的物理稳定性OBs。小角中子散射实验的物理证据支持隔离OBs的程序的完整性。脂肪酶活性的抑制在确定脂质组中是至关重要的。有确凿的证据表明，后者主要是磷脂酰胆碱（约60%）和磷脂酰肌醇（约20%），以及各种其他头部基团（约20%）。表面单层的脂肪酸谱包括棕榈酸、亚油酸和油酸（2：1：0.25，1H NMR），仅具有痕量的其他脂肪酸（C24：0、C22：0、C18：0、C18：3、C16：2;通过MS）。蛋白质组富含油质蛋白（78%），其余由钙蛋白和固醇蛋白组成。这些数据足够详细，可以更新这种细胞器的理解模型，并可用于在分子生物学，生物技术和食品工业应用的范围内使用这些组件。在这项研究中所使用的技术分析脂质体抛出一个新的光对植物细胞室的脂质分布。本文的在线版本（doi：10.1007/s12154-012-0090-1）包含补充材料，可供授权用户使用。
In this paper we report the molecular profiling, lipidome and proteome, of the plant organelle known as an oil body (OB). The OB is remarkable in that it is able to perform its biological role (storage of triglycerides) whilst resisting the physical stresses caused by changes during desiccation (dehydration) and germination (rehydration). The molecular profile that confers such extraordinary physical stability on OBs was determined using a combination of 31P/1H nuclear magnetic resonance (NMR), high-resolution mass spectrometry and nominal mass-tandem mass spectrometry for the lipidome, and gel-electrophoresis-chromatography-tandem mass spectrometry for the proteome. The integrity of the procedure for isolating OBs was supported by physical evidence from small-angle neutron-scattering experiments. Suppression of lipase activity was crucial in determining the lipidome. There is conclusive evidence that the latter is dominated by phosphatidylcholine (∼60 %) and phosphatidylinositol (∼20 %), with a variety of other head groups (∼20 %). The fatty acid profile of the surface monolayer comprised palmitic, linoleic and oleic acids (2:1:0.25, 1H NMR) with only traces of other fatty acids (C24:0, C22:0, C18:0, C18:3, C16:2; by MS). The proteome is rich in oleosins (78 %) with the remainder being made up of caleosins and steroleosins. These data are sufficiently detailed to inform an update of the understood model of this organelle and can be used to inform the use of such components in a range of molecular biological, biotechnological and food industry applications. The techniques used in this study for profiling the lipidome throw a new light on the lipid profile of plant cellular compartments. The online version of this article (doi:10.1007/s12154-012-0090-1) contains supplementary material, which is available to authorized users.