EPIDEMIOLOGIC APPLICATION OF A STANDARDIZED RIBOTYPE SCHEME FOR VIBRIO-CHOLERAE 01
EPIDEMIOLOGIC APPLICATION OF A STANDARDIZED RIBOTYPE SCHEME FOR VIBRIO-CHOLERAE 01
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DOI：
10.1128/jcm.31.9.2474-2482.1993
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发表时间：
1993-09-01
影响因子：
9.4
通讯作者：
WACHSMUTH, K
中科院分区：
文献类型：
--
作者：
POPOVIC, T;BOPP, C;WACHSMUTH, K
根据过去60年来在35个国家和美国14个州分离的214种人类和环境菌株的数据，提出了27种不同BglI核糖体型和霍乱弧菌O 1菌株亚型的标准化方案。所获得的核糖型模式是可重复的，并且随时间推移是稳定的。在16株经典生物型菌株中观察到7种不同但非常相似的核糖体型（1a至1g）。在El Tor生物型的198株霍乱弧菌01菌株中鉴定了20种核糖体型和亚型。在导致目前第七次大流行的菌株中发现了六种不同的模式。核糖体8型的菌株仅起源于中非国家，而核糖体3型的菌株主要起源于亚洲和太平洋岛屿。最广泛分布的菌株是核糖体型6的菌株，其被细分为三个非常相似但仍可区分的亚型。目前拉丁美洲的流行病是由核糖体5型菌株引起的。这种核糖体型的菌株分离自其他几个地理位置，但可以通过其他分子方法与拉丁美洲菌株区分开来。与两个记录的环境水库的菌株表现出三种不同的核糖体型模式;那些从吃美国海湾沃茨的食物的患者分离的核糖体型2，而与澳大利亚东北部河流的菌株的核糖体型9和10。源自拉丁美洲和美国墨西哥湾沿岸的非产毒性霍乱弧菌01菌株未形成特定的核糖体型簇。核糖分型与其他定义明确的方法相结合可以帮助流行病学调查，帮助追踪菌株的运动并确定其地理来源。
A standardized scheme of 27 different BglI ribotypes and subtypes of Vibrio cholerae O1 strains is proposed on the basis of data from 214 human and environmental strains isolated in 35 countries and 14 U.S. states over the past 60 years. The ribotype patterns obtained are reproducible and stable over time. Seven different but very similar ribotypes (1a to 1g) were observed among 16 strains of the classical biotype. Twenty ribotypes and subtypes were identified among 198 V. cholerae 01 strains of the El Tor biotype. Six different patterns were found among the strains causing the current seventh pandemic. Strains of ribotype 8 originated only in central African countries, while those of ribotype 3 originated mainly in Asia and the Pacific Islands. The most widely distributed strains were those of ribotype 6, which was subdivided into three very similar but still distinguishable subtypes. The present Latin American epidemic is caused by strains of ribotype 5. Strains of this ribotype were isolated from several other geographic locations but can be differentiated from the Latin American strains by other molecular methods. Strains associated with two documented environmental reservoirs exhibited three distinct ribotype patterns; those isolated from patients who ate food from the U.S. Gulf waters were all of ribotype 2, while the strains related to the northeast Australian rivers were of ribotypes 9 and 10. Nontoxigenic V. cholerae 01 strains originating in latin America and the U.S. Gulf Coast did not form a specific cluster of ribotypes. Ribotyping in combination with other well-defined methods can assist in epidemiologic investigations, helping to trace the movement of strains and to identify their geographic origins.