The scale of substratum topographic features modulates proliferation of corneal epithelial cells and corneal fibroblasts
The scale of substratum topographic features modulates proliferation of corneal epithelial cells and corneal fibroblasts
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DOI：
10.1002/jbm.a.30744
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发表时间：
2006-10-01
影响因子：
4.9
通讯作者：
Murphy, C. J.
中科院分区：
文献类型：
--
作者：
Liliensiek, S. J.;Campbell, S.;Murphy, C. J.
角膜是由不同类型的细胞组成的复杂组织，包括角膜上皮细胞和角膜细胞。这些细胞类型中的每一种都直接暴露于来自基底膜或周围细胞外基质的丰富的纳米级形貌。纳米尺度的形貌已被证明会影响细胞行为，包括取向、排列、分化、迁移和增殖。我们研究了SV 40转化的人角膜上皮细胞（SV 40-HCECs）、原代人角膜上皮细胞（HCECs）和原代角膜成纤维细胞的增殖是否受到基质地形特征尺度的影响。使用基底膜特征尺寸作为我们的指导和角膜基质的胶原原纤维的已知尺寸（20-60 nm），我们制造了聚氨酯模制基底，其包含在400至4000 nm的间距（间距=脊宽度+槽宽度）上在200-2000 nm的范围内的各向异性特征尺寸。将六个图案化区域中的每一个分开的平面区域用作控制表面。原发性角膜和SV 40-HCEC增殖直接响应于降低至200 nm的纳米级形貌而降低。与角膜上皮细胞相反，角膜成纤维细胞在5天时与平面对照相比，对任何地形的反应都没有表现出显著不同。然而，在培养14天后，在最小特征尺寸上观察到增殖减少。这些实验的结果与理解角膜内细胞增殖和分化的控制中涉及的潜在机制有关。(c)2006 Wiley Periodicals，Inc.
The cornea is a complex tissue composed of different cell types, including corneal epithelial cells and keratocytes. Each of these cell types are directly exposed to rich nanoscale topography from the basement membrane or surrounding extracellular matrix. Nanoscale topography has been shown to influence cell behaviors, including orientation, alignment, differentiation, migration, and proliferation. We investigated whether proliferation of SV40-transformed human corneal epithelial cells (SV40-HCECs), primary human corneal epithelial cells (HCECs), and primary corneal fibroblasts is influenced by the scale of topographic features of the substratum. Using basement membrane feature sizes as our guide and the known dimensions of collagen fibrils of the corneal stroma (20-60 nm), we fabricated polyurethane molded substrates, which contain anisotropic feature sizes ranging from 200-2000 nm on pitches ranging from 400 to 4000 nm (pitch = ridge width + groove width). The planar regions separating each of the six patterned regions served as control surfaces. Primary corneal and SV40-HCEC proliferation decreased in direct response to decreasing nanoscale topographies down to 200 nm. In contrast to corneal epithelial cells, corneal fibroblasts did not exhibit significantly different response to any of the topographies when compared with planar controls at 5 days. However, decreased proliferation was observed on the smallest feature sizes after 14 days in culture. Results from these experiments are relevant in understanding the potential mechanisms involved in the control of proliferation and differentiation of cells within the cornea. (c) 2006 Wiley Periodicals, Inc.