Fiber-aligned polymer scaffolds for rotator cuff repair in a rat model
Fiber-aligned polymer scaffolds for rotator cuff repair in a rat model
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DOI：
10.1016/j.jse.2011.10.021
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发表时间：
2012-02-01
影响因子：
3
通讯作者：
Bernstein, Joseph
中科院分区：
文献类型：
--
作者：
Beason, David P.;Connizzo, Brianne K.;Bernstein, Joseph
背景：近年来，肩袖肌腱撕裂的修复技术有所进步；尽管如此，失败率仍然很高。尽管有各种各样的移植材料可用于修复增强，但还没有在体内使用纤维排列支架的生物力学研究。本研究的目的是评估纤维排列的纳米纤维聚合物支架作为大鼠肩袖损伤模型中潜在的治疗递送载体的功效。材料与方法：采用静电纺丝法制备有牺牲纤维和无牺牲纤维的支架，植入增强大鼠冈上肌修复。没有支架增加的修复也进行了作为对照。术后4周和8周处死大鼠，对修复情况进行组织学和生物力学评价。结果：两种支架配方均保持原位，在损伤和修复后4周和8周，缺乏牺牲纤维的支架有更明显的细胞浸润和定植。支架标本的横截面积比对照组大。生物力学测试显示各组之间的结构特性没有显著差异。在支架组中，材料的一些表观性能明显降低。这些减少是由于横截面积的增加，很可能是由于植入支架材料的额外厚度造成的。2个支架组间无差异。结论：在大鼠肩袖修复中，手术植入覆盖纤维定向支架对冈上肌腱的结构特性无不良影响，验证了该模型作为靶向递送平台的可行性。证据水平：综述文章。(C) 2012年《肩关节外科杂志》理事会。
Background: Repair techniques of rotator cuff tendon tears have improved in recent years; nonetheless, the failure rate remains high. Despite the availability of various graft materials for repair augmentation, there has yet to be a biomechanical study using fiber-aligned scaffolds in vivo. The objective of this study was to evaluate the efficacy of fiber-aligned nanofibrous polymer scaffolds as a potential treatment-delivery vehicle in a rat rotator cuff injury model.Materials and methods: Scaffolds with and without sacrificial fibers were fabricated via electrospinning and implanted to augment supraspinatus repair in rats. Repairs without scaffold augmentation were also performed to serve as controls. Rats were sacrificed at 4 and 8 weeks postoperatively, and repairs were evaluated histologically and biomechanically.Results: Both scaffold formulations remained in place, with more noticeable cellular infiltration and colonization at 4 and 8 weeks after injury and repair for scaffolds lacking sacrificial fibers. Specimens with scaffolds were larger in cross-sectional area compared with controls. Biomechanical testing revealed no significant differences in structural properties between the groups. Some apparent material properties were significantly reduced in the scaffold groups. These reductions were due to increases in cross-sectional area, most likely caused by the extra thickness of the implanted scaffold material. No differences were observed between the 2 scaffold groups.Conclusions: No adverse effect of surgical implantation of overlaid fiber-aligned scaffolds on structural properties of supraspinatus tendons in rat rotator cuff repair was demonstrated, validating this model as a platform for targeted delivery.Level of evidence: Review Article. (C) 2012 Journal of Shoulder and Elbow Surgery Board of Trustees.