QTL Mapping for Cucumber Fruit Size and Shape with Populations from Long and Round Fruited Inbred Lines
QTL Mapping for Cucumber Fruit Size and Shape with Populations from Long and Round Fruited Inbred Lines
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长圆果自交系群体黄瓜果实大小和形状的 QTL 定位
DOI：
10.1016/j.hpj.2020.04.004
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发表时间：
2020-05-01
影响因子：
5.7
通讯作者：
Ren, Zhonghai
中科院分区：
文献类型：
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作者：
Gao, Zhihui;Zhang, Haiyang;Ren, Zhonghai
为了深入了解黄瓜果实大小和形状的分子和遗传基础，我们对华北型自交系CNS21与华南型自交系RNS7杂交的F-2和BC1F1群体进行了数量性状位点（QTL）定位。种群在两个发育阶段，花期子房和商品果（未成熟果）进行评估。6个性状共检测到13个主效qtl (R-2 > 10%)，其中果形指数2.1解释了50%以上的表型变异。所有qtl均分布在染色体1和2上。我们构建了一个物理地图，其中包含了几乎所有的qtl及其在以前研究中的物理位置。在果实大小和形状方面，定位于chr1和chr6的qtl数量最多，定位于chr2和chr7的qtl数量最少。每条染色体上至少存在一个一致区域。此外，通过比较chr3的一致区序列，预测了长果和圆果自交系之间的3个候选基因，该区域已经检测到许多可销售果实长度的qtl。研究结果为黄瓜果实大小和形状的qtl和分子标记的开发提供了基础。
To gain insight into the molecular and genetic basis of fruit size and shape in cucumber, we conducted Quantitative Trait Locus (QTL) mapping with F-2 and BC1F1 populations derived from a cross between the Northern-China type inbred line CNS21 and the Southern-China type inbred line RNS7. Populations were evaluated during two developmental stages, ovary at anthesis and commercial fruit (immature fruit). A total of 13 major-effect QTLs (R-2 > 10%) were detected for six traits, and one of them, fruit shape index 2.1, explained more than 50% of phenotypic variation. All QTLs distributed on chromosome (chr) 1 and 2. We constructed a physical map containing almost all of the QTLs with their physical location from previous studies. For fruit size and shape, the highest number of QTLs were mapped on chr1 and chr6, and the fewest number were mapped on chr2 and chr7. At least one consensus region was presented on each chromosome. In addition, three candidate genes were predicted between the long-fruited and round-fruited inbred lines by comparing the sequences of the consensus region of chr3, where many QTLs for marketable fruit length had been detected. Our results provide a base for additional QTLs and molecular markers for fruit size and shape in cucumber breeding.