Genome-wide identification and expression analyses of the calmodulin and calmodulin-like proteins reveal their involvement in stress response and fruit ripening in papaya
Genome-wide identification and expression analyses of the calmodulin and calmodulin-like proteins reveal their involvement in stress response and fruit ripening in papaya
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钙调蛋白和钙调蛋白样蛋白的全基因组鉴定和表达分析揭示了它们参与木瓜的应激反应和果实成熟
DOI：
10.1016/j.postharvbio.2018.04.010
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发表时间：
2018-09-01
影响因子：
7
通讯作者：
Zhu, Xiaoyang
中科院分区：
文献类型：
--
作者：
Ding, Xiaochun;Zhang, Linpeng;Zhu, Xiaoyang
钙(Ca~(2+))是植物体内重要的第二信使，参与植物的各种发育和适应过程。钙调素(CaM)和类钙调素(CML)蛋白是控制多种细胞功能的主要钙离子感受器。本研究在番木瓜基因组中鉴定出41个编码CaM和CML蛋白的基因，其中3个为CaM，其余为CML。序列比对、基因结构和系统发育分析表明，所有的CaM/CML都含有EF-Hand结构域，而不含其他功能结构域，并且CaM蛋白非常保守，而CML蛋白则表现出序列多样性和结构多样性。启动子分析鉴定了CpCaM/CML基因启动子区域中与植物生长发育、激素反应、光反应、胁迫反应和转录增强相关的不同类型的顺式元件。基因表达谱分析表明，CaM/CMLS在不同组织类型、不同果实发育阶段和不同贮藏条件下的表达谱存在差异。CML16、CML17.1、CML24和CML36等几组CaM/CML基因受高温和低温胁迫的正向或负向调控，表明它们可能在温度胁迫适应中发挥作用。值得注意的是，一些CaM/CML基因受到乙烯(乙烯利和1-MCP处理)的严格调控，无论是正调控还是负调控，如CaM7、CML15、CML16、CML17.1、CML37和CML46。这些结果表明，CaM和CML基因家族可能在果实发育、对温度胁迫的响应和果实成熟过程中发挥重要作用。
Calcium (Ca2+) is an important second messenger involved in diverse developmental and adaptive processes in plants. Calmodulin (CaM) and calmodulin-like (CML) proteins are primary Ca2+ sensors that control diverse cellular functions. In this study, 41 genes encoding CaM and CML proteins were identified in the papaya genome, three of which were CaM and others were CML. Sequence alignment, gene structural and phylogenetic analyses revealed that all CaM/CMLs contained the EF-hand, but not other functional domains, and CaM proteins were quite conservative while CML proteins exhibited sequence diversity and structural multiformity. Promoter analysis identified different types of cis-elements related to plant growth and development, hormone response, light response, stress response and transcriptional enhancement in promoter regions of CpCaM/CML genes. Gene expression analysis showed distinctive expression profiles of the CaM/CMLs in different tissue types, different fruit developmental stages and different fruit storage conditions. Several groups of CaM/CML genes were positively or negatively regulated by high and low temperature stresses, such as CML16, CML17.1, CML24 and CML36, indicating that they may play a role in temperature stress adaption. Notably, some CaM/CML genes were rigorously regulated by ethylene (ethephon and 1-MCP treatment), either in a positive or a negative manner, such as CaM7, CML15, CML16, CML17.1, CML37 and CML46. All these results indicated that CaM and CML gene families might play important roles in fruit development, in response to temperature stresses and in fruit ripening process.