Cutting edge: The tumor counterattack hypothesis revisited: Colon cancer cells do not induce T cell apoptosis via the Fas (CD95, APO-1) pathway
Cutting edge: The tumor counterattack hypothesis revisited: Colon cancer cells do not induce T cell apoptosis via the Fas (CD95, APO-1) pathway
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DOI：
10.4049/jimmunol.164.10.5023
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发表时间：
2000-05-15
影响因子：
4.4
通讯作者：
Bonnotte, B
中科院分区：
文献类型：
--
作者：
Favre-Felix, N;Fromentin, A;Bonnotte, B
反击假说认为，癌细胞表达Fas配体（FasL）并能够杀死表达Fas的肿瘤浸润活化T细胞，这一假说得到了快速表达的人类结肠癌细胞系杀死Jurkat细胞的报道的支持。通过使用一种改进的细胞毒性试验，其中可溶性Fast和fasl转染的KFL9细胞作为阳性对照，我们发现7种人类结肠癌细胞系中没有一种诱导表达fasl的靶细胞系Jurkat和L1210-Fas细胞凋亡。此外，在共培养实验中，癌细胞单层并不抑制表达fas的淋巴样细胞的生长。虽然在结肠癌细胞系的提取物中检测到Fast mRNA和蛋白，但流式细胞术和共聚焦显微镜无法检测到肿瘤细胞表面的蛋白。这些结果表明，癌细胞对肿瘤浸润性T淋巴细胞的反击可能不能解释对肿瘤细胞的免疫耐受。
The counterattack hypothesis, suggesting that cancer cells express Fas ligand (FasL) and are able to kill Fas-expressing tumor-infiltrating activated T cells, was supported by reports of the killing of Jurkat cells by Fast-expressing human colon cancer cell lines. Through the use of an improved cytotoxic assay in which soluble Fast and FasL-transfected KFL9 cells were used as positive controls, we show that none of seven human colon cancer cell lines induce apoptosis of two Fas-expressing target cell lines, Jurkat and L1210-Fas cells. Moreover, in coculture experiments, cancer cell monolayers do not inhibit the growth of Fas-expressing lymphoid cells. Although Fast mRNA and protein were detected in the extracts of the colon cancer cell lines, how cytometry and confocal microscopy failed to detect the protein on the surface of tumor cells. These results suggest that the counterattack of tumor-infiltrating T lymphocytes by cancer cells may not account for immune tolerance toward tumor cells.