Enhanced polarization of embryonic hippocampal neurons on micron scale electrospun fibers
Enhanced polarization of embryonic hippocampal neurons on micron scale electrospun fibers
复制标题
DOI：
10.1002/jbm.a.32471
复制
发表时间：
2010-03-15
影响因子：
4.9
通讯作者：
Schmidt, Christine E.
中科院分区：
文献类型：
--
作者：
Lee, Jae Young;Bashur, Chris A.;Schmidt, Christine E.
电纺纤维已被广泛应用于人工组织工程支架材料。特别是，比细胞体更小的纤维已被广泛用于模拟自然细胞外基质(ECM)和探索各种细胞类型的特定反应。我们从轴突形成(即极化)和轴突方向的角度，研究了不同的聚乳酸-乙醇酸(PLGA)纤维特征对胚胎发育早期海马神经元的影响。我们生产的PLGA纤维的平均直径从0.44微米到2.2微米不等，纤维的排列程度不同(角度标准偏差为16-58度)。培养22h后，生长在PLGA纤维上的胚胎海马神经元显示出更多的轴突形成，比涂有自旋涂层的光滑PLGA膜多30-50%。这种改善与纤维直径和排列无关；然而，在较小的纤维和排列较多的纤维上观察到略微更多的偏振。此外，与生长在自旋涂层PLGA膜上的细胞相比，不同PLGA纤维的极化胚胎海马神经元的平均轴突长度没有显著差异。这些发现表明，亚细胞直径的纤维刺激轴突的初始建立并引导轴突延伸的方向；然而，这些纤维似乎并不影响轴突的总长度。这些信息将对理解亚细胞特征在神经元发育中的作用以及设计用于神经组织接口的生物材料具有价值。(C)2009年威利期刊公司《生物医学杂志》92a：1.398-1406年，2010年
Electrospun fibers have been fabricated for wide use as artificial tissue engineering scaffolds. In particular, fibers smaller than a cell body have been extensively employed to mimic natural extracellular matrix (ECM) and to explore specific responses by various cell types. We investigated the effects of various poly(lactic acid-co-glycolic acid) (PLGA) fiber features on embryonic hippocampal neurons in the early developmental stages in terms of initial axon formation (i.e., polarization) and axon orientation. We produced PLGA fibers that have average diameters ranging from 0.44 mu m to 2.2 mu m and different degrees of fiber alignment (16-58 degrees in angular standard deviation). After 22 h in culture, embryonic hippocampal neurons grown on PLGA fibers exhibited more axon formation with a 30-50% increase over those on spin-coated smooth PLGA films. This improvement was independent of fiber diameter and alignment; however, slightly more polarization was observed on the smaller fibers and the more aligned fibers. In addition, average axon length of the polarized embryonic hippocampal neurons was not significantly different among the PLGA fibers when compared with cells grown on spin-coated PLGA films. These findings suggest that fibers of subcellular diameters stimulate initial axon establishment and guide the direction of axonal extension; however, these fibers do not appear to affect the overall axon length. This information will be valuable in understanding the roles of subcellular features on neuron development and for the design of biomaterials for neural tissue interfacing. (C) 2009 Wiley Periodicals, Inc. J Biomed Mater Res 92A: 1.398-1406, 2010