Genetic transformation of Borrelia burgdorferi.
Genetic transformation of Borrelia burgdorferi.
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DOI：
10.1002/9780471729259.mc12c04s20
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发表时间：
2011-02-01
影响因子：
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通讯作者：
Skare, Jon T
中科院分区：
文献类型：
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作者：
Hyde, Jenny A;Weening, Eric H;Skare, Jon T
开发强大的遗传工具来操纵莱姆病的病原体伯氏疏螺旋体，现在允许研究人员在实验性莱姆病疏螺旋体病的背景下评估单个基因的作用。本单元致力于描述可用于B的分子遗传学分析的实验方法。burgdorferi，重点在于培养、电转化、所需突变体的选择和获得突变体的遗传互补。其目的是提供一个共识协议，该协议封装了B当前采用的方法。Burgdorferi研究界并描述了在使用这些致病螺旋菌时必须考虑的相关问题。
The development of robust genetic tools to manipulate Borrelia burgdorferi, the etiologic agent of Lyme disease, now allows investigators to assess the role(s) of individual genes in the context of experimental Lyme borreliosis. This unit is devoted to the description of experimental approaches that are available for the molecular genetic analysis of B. burgdorferi with an emphasis on cultivation, electrotransformation, selection of desired mutants, and genetic complementation of acquired mutants. The intent is to provide a consensus protocol that encapsulates the methodologies currently employed by the B. burgdorferi research community and describe pertinent issues that must be accounted for when working with these pathogenic spirochetal bacteria.