A resource for cell line authentication, annotation and quality control
A resource for cell line authentication, annotation and quality control
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DOI：
10.1038/nature14397
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发表时间：
2015-04-16
期刊：
影响因子：
64.8
通讯作者：
Neve, Richard M.
中科院分区：
文献类型：
--
作者：
Yu, Mamie;Selvaraj, Suresh K.;Neve, Richard M.
细胞系鉴定错误、污染和注释不良影响科学的可重复性。在这里，我们概述了检测或避免交叉污染的简单措施，提出了与短串联重复序列和单核苷酸多态性谱相关的细胞系注释框架，并提供了同义细胞系的目录。该资源将使我们的社区能够消除使用错误识别的线路并生成可信的基于细胞的数据。
Cell line misidentification, contamination and poor annotation affect scientific reproducibility. Here we outline simple measures to detect or avoid cross-contamination, present a framework for cell line annotation linked to short tandem repeat and single nucleotide polymorphism profiles, and provide a catalogue of synonymous cell lines. This resource will enable our community to eradicate the use of misidentified lines and generate credible cell-based data.