UV resonance Raman studies of molecular structure and dynamics: applications in physical and biophysical chemistry.
UV resonance Raman studies of molecular structure and dynamics: applications in physical and biophysical chemistry.
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DOI：
10.1146/annurev.pc.39.100188.002541
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发表时间：
1988
影响因子：
14.7
通讯作者：
S. Asher
中科院分区：
文献类型：
--
作者：
S. Asher
对分子结构和动力学的基本理解需要理论和实验之间的紧密耦合。一个最终目标是利用分子结构和动力学的理解来预测分子性质，如化学反应性和本体性质，如相行为和材料强度。科学进步的主要动力往往是新实验技术的发展，这些技术能够敏锐地洞察复杂系统中的简单现象。这对于研究大的多原子分子尤其重要，并且对于控制生命过程的生物学和生物化学中研究的大分子至关重要。本文综述了紫外共振拉曼光谱（UVRR）作为一种研究小分子和大分子（如多肽和蛋白质）的分子结构和动力学的新技术的最新进展。这项最新的工作是在1975年开创性的紫外拉曼核酸研究之后进行的，该研究使用257 nm的倍频Ar激光激发（1-4），以及齐格勒和阿尔布雷希特开创性的氮染料激光预共振拉曼苯衍生物研究，该研究为苯衍生物和其他芳烃的真共振拉曼研究奠定了基础（5-8）。这项工作是继
A fundamental understanding of molecular structure and dynamics requires a close coupling between theory and experiment. One ultimate objective is to utilize the understanding of molecular structure and dynamics to predict both molecular properties such as chemical reactivity and bulk properties such as phase behavior and material strengths. Often the major driving force for progress in science is the development of new experimental techniques capable of incisive glimpses into simple phenomena in complex systems. This is especially important for studies of large polyatomic molecules, and is crucial for the macromolecules studied in biology and biochemistry that control life processes. This review outlines the recent progress in the development of UV resonance Raman spectroscopy (UVRR) as a new technique for the study of molecular structure and dynamics for both small molecules and larger molecules such as polypeptides and proteins. This recent work follows the pioneering UV Raman nucleic acid studies in 1975, which used frequency doubled Ar laser excitation at 257 nm (1-4), and Ziegler & Albrecht’s pioneering nitrogen-dye laser preresonance Raman benzene derivative studies, which set the stage for true resonance Raman investigations of benzene derivatives and other aromatics (5-8). This work was followed