Relationship between the expression of Rab family GTPase and neuropeptide hormones in the brain of Bombyx mori. Histochem.
Relationship between the expression of Rab family GTPase and neuropeptide hormones in the brain of Bombyx mori. Histochem.
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家蚕脑中 Rab 家族 GTPase 表达与神经肽激素的关系
DOI：
10.1007/s00418-012-1021-5
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Takeda M.
中科院分区：
文献类型：
--
作者：
Uno T.;Sakamoto K;Isoyama Y;Hiragaki S;Uno Y;Kanamaru K;Yamagata H;Takagi M;Mizoguchi A;Takeda M.
Rab蛋白是在囊泡运输中起重要作用的小GTP酶。在这项研究中，我们研究了Rab蛋白和神经肽激素在家蚕脑中的表达。我们制造了抗B的抗体。moriRab1和Rab14。B.脑组织样品的免疫印迹。Mori显示每种抗体的单一条带。Rab1和Rab14在B.死亡仅限于大脑间部和前脑背外侧区的神经元。Rab1、Rab7和Rab14与家蚕毒素共定位。Rab1和Rab7与羽化激素共定位。Rab1与促前胸腺激素共定位。这些结果提示Rab1、Rab7和Rab14可能参与了脑内神经肽的转运。这是首次报道Rab蛋白对昆虫分泌不同神经肽的特异性。
Rab proteins are small GTPases that play essential roles in vesicle transport. In this study, we examined the expression of Rab proteins and neuropeptide hormones in the brain of the silkworm,Bombyx mori. We produced antibodies againstB. moriRab1 and Rab14 in rabbits. Immunoblotting of samples of brain tissue fromB. morirevealed a single band for each antibody. Rab1 and Rab14 immunohistochemical labeling in the brain ofB. moriwas restricted to neurons of the pars intercerebralis and dorsolateral protocerebrum. Rab1, Rab7 and Rab14 co-localized with bombyxin. Rab1 and Rab7 co-localized with eclosion hormone. Rab1 co-localized with prothoracicotropic hormone. These results suggest that Rab1, Rab7 and Rab14 may be involved in neuropeptide transport in the brain ofB. mori.This is the first report on the specificity of Rab proteins for the secretion of different neuropeptides in insects.