NUCLEOSIDE ANALYSIS OF DNA FROM TRYPANOSOMA-BRUCEI AND TRYPANOSOMA-EQUIPERDUM
NUCLEOSIDE ANALYSIS OF DNA FROM TRYPANOSOMA-BRUCEI AND TRYPANOSOMA-EQUIPERDUM
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DOI：
10.1016/0166-6851(88)90163-6
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发表时间：
1988-11-01
影响因子：
1.5
通讯作者：
BORST, P
中科院分区：
文献类型：
--
作者：
CROZATIER, M;DEBRIJ, RJ;BORST, P
我们用胰腺DNase I、核酸酶P1和牛碱性磷酸酶的组合消化锥虫DNA，并在Supelcosil LC-18-S色谱柱上用高效液相色谱分离得到的核苷。我们在布氏锥虫和马锥虫种群中都发现了不到0.1%的异常核苷，而此前有报道称，在马锥虫总核苷的1.3%中，异常核苷取代了DC。我们的结果与先前的观点一致，即修饰布鲁氏毛滴虫变种特异性表面糖蛋白基因的非活性端粒表达部位只影响很小一部分总DNA。
We have digested trypanosome DNA with a combination of pancreatic DNase I, nuclease P1 and bovine alkaline phosphatase and fractionated the resulting nucleosides on a Supelcosil LC-18-S column by high presence liquid chromatography. We find less than 0.1% unusual nucleosides, both in Trypanosoma brucei and in a Trypanosoma equiperdum stock, in contrast to a previous report of an unusual nucleoside replacing dC at 1.3% of total nucleosides in T. equiperdum. Our results agree with previous suggestions that the modification of inactive telomeric expression sites for variant-specific surface glycoprotein genes in T. brucei only affects a very small fraction of the total DNA.