Histochemical, enzymatic, and contractile proper of skeletal muscles of three anuran amphibians.
Histochemical, enzymatic, and contractile proper of skeletal muscles of three anuran amphibians.
复制标题
三种无尾两栖动物骨骼肌的组织化学、酶学和收缩特性。
DOI：
--
复制
发表时间：
1983
影响因子：
--
通讯作者：
A. F. Bennett
中科院分区：
文献类型：
--
作者：
R. Putnam;A. F. Bennett
罗伯特·普特南，安达尔伯特F.班尼特三种无尾两栖动物骨骼肌的组织化学、酶和收缩特性。Am. J. Physiol.244（Regulatory Integrative Comp.Physiol.13）：R558-R567，1983年。比较了蟾蜍、尖音蛙和非洲爪蟾几种后肢肌肉的组织化学、酶和收缩特性。在组织化学染色的基础上，描述了三种纤维类型：I）快收缩，糖酵解（FG）大（140 μ m直径）纤维，其对肌球蛋白腺苷三磷酸酶和cw-甘油磷酸脱氢酶染色深，对琥珀酸脱氢酶染色浅; 2）快速收缩、氧化、糖酵解（FOG）-较小（70 μ m）纤维，其对所有三种染色剂染色深;和3）主音-对于所有三种污渍轻微染色的小（75 μ m）纤维。张力纤维被限制在外围纤维层或口袋中。肌肉的大部分由80%的FG和20%的FOG纤维组成。这种纤维类型的配置文件是相似的运动肌肉在一个给定的物种和物种之间。尽管这种组织化学相似的纤维配置文件，生化酶活性（柠檬酸合酶和乳酸脱氢酶）变化与有机体代谢活动。测量等长收缩时间和半松弛时间以评估收缩动力学。此外，还测量了强直期间张力上升的最大速率。这些收缩特性因物种而异，蟾蜍的肌肉收缩速度比蛙和爪蟾慢。来自三个物种的给定肌肉对重复疲劳刺激有类似的反应，尽管蟾蜍肌肉积累的乳酸是蛙和爪蟾的一半。这些数据表明，蟾蜍的肌肉有一个较低的耐受性乳酸积累，需要较少的能量来产生等长张力比其他两个物种的肌肉。无尾两栖动物的有机体运动和代谢模式的差异与酶和收缩特性的数量差异相关，而不是与肌纤维类型或比例的变化。
PUTNAM,ROBERT W., ANDALBERT F. BENNETT. Histochemical, enzymatic, and contractile properties of skeletal muscles of three anuran amphibians. Am. J. Physiol. 244 (Regulatory Integrative Comp. Physiol. 13): R558-R567, 1983.-The histochemical, enzymatic, and contractile properties of several hindlimb muscles from Bufo boreas, Rana pipiens, and Xenopus Zaeuis were compared. On the basis of histochemical staining, three fiber types are described: I) fast-twitch, glycolytic (FG)large (140 pm diam) fibers that stain darkly for myosin adenosine triphosphatase and cw-glycerophosphate dehydrogenase and lightly for succinate dehydrogenase; 2) fast-twitch, oxidative, glycolytic (FOG)-smaller (70 ,um) fibers that stain darkly for all three stains; and 3) tonic -small (75 pm) fibers that stain lightly for all three stains. Tonic fibers are restricted to peripheral fiber layers or pockets. The bulk of the muscle is composed of 80% FG and 20% FOG fibers by area. This fiber-type profile is similar among locomotory muscles within a given species and among species. Despite this histochemical similarity in fiber profile, biochemical enzymatic activity (citrate synthase and lactate dehydrogenase) varies in correlation with organismal metabolic activity. The isometric twitch contraction time and half-relaxation time were measured to assess contractile kinetics. In addition, the maximum rate of tension rise during a tetanus was measured. These contractile properties varied among species, with Bufo muscles contracting more slowly than those of Rana and Xenopus. A given muscle from the three species had a similar response to repetitive fatiguing stimulation, although Bufo muscles accumulated half as much lactate as those of Rana and Xenopus. These data suggest that the muscles of Bufo have a lower tolerance to lactate accumulation and require less energy to generate isometric tension than the muscles of the other two species. The differences in organismal locomotory and metabolic patterns in anuran amphibians are correlated with quantitative differences in enzymatic and contractile properties, and not with variations in muscle fiber types or proportion.