Comparative analyses of reproductive characteristics of functional sex reversal male gynogenetic red crucian carp and ordinary male red crucian carp
Comparative analyses of reproductive characteristics of functional sex reversal male gynogenetic red crucian carp and ordinary male red crucian carp
复制标题
功能性性别逆转雄性雌核红鲫与普通雄性红鲫繁殖特性的比较分析
DOI：
10.1016/j.aquaculture.2019.06.013
复制
发表时间：
2019
期刊：
影响因子：
4.5
通讯作者：
Liu Shaojun
中科院分区：
文献类型：
--
作者：
Hu Fangzhou;Fan Jingjing;Luo Kaikun;Zhou Yunfan;Wu Chang;Luo Linlin;Wang Shi;Tao Min;Zhang Chun;Chen Bo;Ma Ming;Liu Shaojun
以100 μg/g饲料的17-α-甲基睾酮（MT）对雌核发育红鲫进行60 d的口服处理，获得了功能性性逆转雄雌核发育红鲫（RGRCC）。在本研究中，我们的目的是探讨MT诱导性逆转是否损害RGRCC的形态和生殖功能。在繁殖季节，随机选择20尾普通红鲫和20尾RGRCC成熟雄鲤，测定其表型性状和繁殖性状。RGRCC与普通男性RCC的形态学特征和精子形态学特征无显著性差异（P> 0.05）。RGRCC和普通雄性RCC的平均体重、性腺指数（GSI）、精子密度、精子体积和受精率分别为265.92和270.71 g、3.74和3.82%、3.04和3.22 × 1010 cells ml-1、8.73和8.87 kg ml-1、84.66%和86.84%。此外，用计算机辅助精子分析技术测定了两种鱼精子的曲线运动速度、平均运动速度、直线运动速度、直线运动速度、运动精子百分率和精子头部侧向位移幅度等6项运动参数，结果表明两种鱼精子运动参数之间差异不显著（P> 0.05）。我们的结论是，生殖能力的RGRCC不受MT治疗。RGRCC可用于获得全雌性RCC，这将增加其在水产养殖中应用的生产潜力。
Functional sex reversal male gynogenetic red crucian carp (RGRCC) were obtained by oral administration of 17-α-methyltestosterone (MT) at a dosage of 100 μg/g diet for 60 days to gynogenetic red crucian carp. In the present study, we aim to investigate whether MT induction sex-reversal impairs morphology and reproductive function of RGRCC. During the reproductive season, 20 mature males of both ordinary red crucian carp (RCC) and RGRCC were randomly chosen to measure the phenotypic traits and reproductive traits. None of the morphological traits and nor sperm traits measured differed significantly between RGRCC and ordinary male RCC (P> .05). The mean body weight, gonadosomatic index (GSI), sperm concentration, sperm volume and fertilization rate of RGRCC and ordinary male RCC, were 265.92 and 270.71 g, 3.74 and 3.82%, 3.04 and 3.22 × 1010cells ml−1, 8.73 and 8.87 kg ml−1, 84.66% and 86.84%, respectively. In addition, six sperm motility parameters including values of curvilinear velocity, average path velocity, straight line velocity, linearity, percentage of motile sperm and amplitude of lateral displacement of the sperm head were assessed by computer assisted sperm analysis and there were not significant differences (P> .05) between these two fishes. We concluded that the reproductive capacities of RGRCC were not affected by MT treatments. RGRCC could be used to obtain all-female RCC which will increase their production potential for application in aquaculture.