CELL TYPE CONVERSION IN ISOLATED PRESTALK AND PRESPORE FRAGMENTS OF THE CELLULAR SLIME MOLD DICTYOSTELIUM DISCOIDEUM
CELL TYPE CONVERSION IN ISOLATED PRESTALK AND PRESPORE FRAGMENTS OF THE CELLULAR SLIME MOLD DICTYOSTELIUM DISCOIDEUM
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细胞粘菌盘基网柄菌分离的前茎和预孢子碎片中的细胞类型转换
DOI：
10.1111/j.1440-169x.1973.00011.x
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发表时间：
1973
期刊：
影响因子：
4.6
通讯作者：
Y. Sakai
中科院分区：
文献类型：
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作者：
Y. Sakai
圆盘蛞蝓的碎片会导致两种类型的分化细胞（前茎细胞和前孢子细胞）之间的转换，以便在每个碎片中重新建立它们的正常比例。通过确定片段中包含的前孢子细胞的比例，对这一过程的动力学进行了定量研究。从前茎细胞到前孢子细胞（在前茎分离物中）的转化仅在约3小时的滞后期后发生，并在孵育7-8小时内完成。相反，从前孢子细胞到前柄细胞（在前孢子分离物中）的转化没有滞后地发生，并在孵育 3-4 小时内完成。这两种类型的转化在低温和200pg/ml放线菌酮或125pg/ml放线菌素D存在下均被抑制。在10mM LiCl存在下，前茎细胞向前孢子细胞的转化被完全抑制，而反向转化完全不受影响。此外，前孢子分离物中的所有细胞最终都转化为前茎细胞。完整的蛞蝓也是如此。
Fragmentation of a slug of D. discoideurn causes the conversion between the two types of differentiated cells (prestalk and prespore cells), in order to reestablish their normal proportion in each fragment. A quantitative study on the kinetics of such a process was made by determining the proportion of the prespore cell contained in a fragment. The conversion from the prestalk cell to the prespore cell (in a prestalk isolate) occured only after a lag phase of about 3 hr and completed within 7-8 h r of incubation. On the contrary, the conversion from the prespore cell to the prestalk cell (in a prespore isolate) occured without a lag and completed within 3-4 hr of incubation. The both types of conversion were inhibited at a low temperature and in the presence of 200pg/ml cycloheximide or 125 pg/ml actinomycin D. The conversion from the prestalk cell to the prespore cell was completely inhibited in the presence of 10 mM LiCl, while the reverse conversion was not affected at all. Moreover, all the cells in a prespore isolate were eventually converted to the prestalk cell. This is also the case with an intact slug.