Novel brevibacillus choshinensis and process for producing protein with use of the microbe as host
Novel brevibacillus choshinensis and process for producing protein with use of the microbe as host
复制标题
新型长信短芽孢杆菌及以该微生物为宿主生产蛋白质的方法
DOI：
--
复制
发表时间：
2004
期刊：
影响因子：
--
通讯作者：
히로시 하나가타
中科院分区：
文献类型：
--
作者：
다카유키 니시죠;히로시 하나가타
BRAY比率的铫子芽孢杆菌（Bacillus Choshi）双烯系统表明胞外蛋白质水解活性非常低，并且在蛋白质分泌和生产能力方面表现出优异的效率。然而，理想的是不仅进一步降低其胞外蛋白水解活性，而且降低蛋白的胞外水解活性的细胞也能实现。此外，在用于蛋白质药物的细菌中，例如细菌，期望容易破碎而不形成孢子。这一问题已经通过对孢子形成相关基因进行灭活和细胞外及细胞内的蛋白酶基因进行克隆和灭活而得到解决。
BRAY ratio Bacillus Choshi norbornene system indicates a remarkably low protein hydrolysis activity of the extracellular, and exhibits excellent efficiency in the ability of the protein secretion and production. However, it is desirable not only to further reduce its extracellular protein hydrolytic activity and reduced protein of extracellular hydrolytic activity of the cell also achieved. Furthermore, in the bacteria to the use for a protein drug, such as, bacteria, it is desired to be easily broken without forming spores. This has been solved by performing the inactivated sporulation-related genes and extracellular and cell for the cloning and in the protease gene inactivated.