MONOCLONALITY IN B-CELL LYMPHOMA DETECTED IN PARAFFIN WAX EMBEDDED SECTIONS USING THE POLYMERASE CHAIN-REACTION
MONOCLONALITY IN B-CELL LYMPHOMA DETECTED IN PARAFFIN WAX EMBEDDED SECTIONS USING THE POLYMERASE CHAIN-REACTION
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DOI：
10.1136/jcp.43.11.888
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发表时间：
1990-11-01
影响因子：
3.4
通讯作者：
MORLEY, AA
中科院分区：
文献类型：
--
作者：
WAN, JH;TRAINOR, KJ;MORLEY, AA
采用聚合酶链反应（PCR）技术，建立了一种在福尔马林固定、石蜡包埋材料中检测B淋巴细胞DNA单克隆的简便方法。切片脱蜡脱水，用水煮沸45分钟提取DNA。采用半巢式PCR扩增免疫球蛋白重链基因V-D-J区。产物经溴化乙锭染色后进行电泳和紫外光观察。26例B细胞淋巴瘤标本24例产生单克隆带，2例无扩增；单克隆性是这种疾病的特异性。7例T细胞淋巴瘤标本无扩增；来自9个反应性淋巴结的标本产生广泛的多克隆物质涂片；12例癌标本无扩增或无多克隆物质产生。由于单克隆的检测是肿瘤疾病的强烈提示，该技术可能在常规诊断中有价值，因为它的速度，简单，适用于固定的，嵌入的材料。
The polymerase chain reaction (PCR) was used to develop a simple technique for detecting monoclonality at the DNA level in B lymphocyte populations in formalin fixed, paraffin wax embedded material. Sections were dewaxed and dehydrated, and the DNA was extracted by boiling in water for 45 minutes. A semi-nested PCR was performed to amplify the V-D-J region of the immunoglobulin heavy chain gene. The product was electrophoresed and viewed under ultraviolet light after ethidium bromide staining. Specimens from 26 B cell lymphomas produced a monoclonal band in 24 cases and no amplification in two cases; monoclonality was specific for this disorder. Specimens from seven T cell lymphomas produced no amplification; specimens from nine reactive nodes produced a broad smear of polyclonal material; and specimens from 12 cases of carcinoma produced either no amplification or polyclonal material. As detection of monoclonality is strongly suggestive of neoplastic disease, this technique is likely to be of value in routine diagnosis, because of its speed, simplicity, and applicability to fixed, embedded material.