Dinucleotide repeat polymorphism at the D9S123 locus (9q31-34).
Dinucleotide repeat polymorphism at the D9S123 locus (9q31-34).
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D9S123 基因座 (9q31-34) 处的二核苷酸重复多态性。
DOI：
10.1093/nar/20.4.934
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发表时间：
1992
影响因子：
14.9
通讯作者：
Gusella,JF
中科院分区：
文献类型：
--
作者：
Kwaitkowski,DJ;Gusella,JF
孟德尔遗传：在五个两代家庭中观察到共同显性遗传。 PCR条件/注释：PCR按照(1)所述进行，在1.5 mM Mg2中进行29个循环，即94℃变性30秒，58℃退火30秒，72℃延伸40秒。使用内部标记，并通过使用 7 Murea 的聚丙烯酰胺凝胶电泳确定等位基因大小。致谢：这项工作得到了 NIH 拨款 HG00324 和 HG00169、肌张力障碍医学研究基金会和国家结节性硬化症协会的支持。参考文献：1) Kwiatkowski, DJ et al.(1992) Genomics 12, 229-240。
Mendelian Inheritance: Co-dominant inheritance was observed in five two-generation families. PCR Conditions/Comments: PCR was performed as described (1), with 29 cycles of denaturation at 94 C for 30 s, annealing at 58 C for 30 s, and extension at 72 C for 40 s in 1.5 mM Mg2. Internal labelling was used, and allele sizes were determined by polyacrylamide gel electrophoresis with 7 Murea. Acknowledgements: Thiswork was supported by NIH grants HG00324 and HG00169, the Dystonia Medical Research Foundation, and the National Tuberous Sclerosis Association. Reference: 1) Kwiatkowski, DJ et al.(1992) Genomics 12, 229-240.