Hydrogen Activates ATP-Binding Cassette Transporter A1-Dependent Efflux Ex Vivo and Improves High-Density Lipoprotein Function in Patients With Hypercholesterolemia: A Double-Blinded, Randomized, and Placebo-Controlled Trial
Hydrogen Activates ATP-Binding Cassette Transporter A1-Dependent Efflux Ex Vivo and Improves High-Density Lipoprotein Function in Patients With Hypercholesterolemia: A Double-Blinded, Randomized, and Placebo-Controlled Trial
复制标题
氢气在体外激活 ATP 结合盒转运蛋白 A1 依赖性流出并改善高胆固醇血症患者的高密度脂蛋白功能：一项双盲、随机和安慰剂对照试验。
DOI：
10.1210/jc.2015-1321
复制
发表时间：
2015-07-01
影响因子：
5.8
通讯作者：
Qin, Shucun
中科院分区：
文献类型：
--
作者：
Song, Guohua;Lin, Quanqiang;Qin, Shucun
上下文
我们在一项前后对照研究中发现，氢（二氢[H2]）可降低潜在代谢综合征患者的血浆低密度脂蛋白（LDL）胆固醇水平，改善高密度脂蛋白（HDL）功能。
目的
本研究的目的是在一项双盲、随机和安慰剂对照试验中进一步表征富氢水（0.9 L/天）对高胆固醇血症患者血浆脂蛋白含量、组成和生物活性的影响及其潜在机制。
设计
这是一项病例对照研究。
设置
背景是中国泰安的周店社区。
患者
共有68例未经治疗的孤立性高胆固醇血症患者被随机分配到饮用富氢水（n = 34）或安慰剂水（n = 34）10周。
结果
从H2组中分离的HDL显示出增强的促进ATP结合盒转运蛋白A1介导的胆固醇体外流出的能力。血浆前-β-HDL水平上调，但血浆HDL-胆固醇水平无变化。此外，其他高密度脂蛋白的功能，在保护LDL氧化，抑制氧化LDL诱导的炎症，并保护内皮细胞氧化LDL诱导的凋亡，都显着改善H2治疗。此外，H2治疗降低血浆总胆固醇（47.06% vs 17.65%）和LDL胆固醇（47.06% vs 23.53%）水平的有效率增加。Western blot分析显示H2组血浆载脂蛋白B100显著降低，载脂蛋白M显著升高。最后，H2处理导致全血浆和HDL颗粒中几种炎症和氧化应激指标的水平显著降低。
结论
H2激活ATP结合盒转运体A1依赖性外排，增强HDL抗动脉粥样硬化功能，并具有有益的降脂作用。目前的研究结果突出了H2在高胆固醇血症和动脉粥样硬化消退中的潜在作用。
CONTEXT
We have found that hydrogen (dihydrogen [H2]) decreases plasma low-density lipoprotein (LDL) cholesterol levels and improves high-density lipoprotein (HDL) function in patients with potential metabolic syndrome in a before-after self-controlled study.
OBJECTIVE
The purpose of this study was to further characterize the effects of H2-rich water (0.9 L/day) on the content, composition, and biological activities of plasma lipoproteins on patients with hypercholesterolemia and their underlying mechanisms in a double-blinded, randomized, and placebo-controlled trial.
DESIGN
This was a case-control study.
SETTING
The setting was the Zhoudian community, Tai'an, China.
PATIENTS
A total of 68 patients with untreated isolated hypercholesterolemia were randomly allocated to either drinking H2-rich water (n = 34) or placebo water (n = 34) for 10 weeks.
RESULTS
HDL isolated from the H2 group showed an increased ability to promote the ATP-binding cassette transporter A1-mediated cholesterol efflux ex vivo. Plasma pre-β-HDL levels were up-regulated although there were no changes in plasma HDL-cholesterol levels. Moreover, other HDL functions, assessed in protection against LDL oxidation, inhibition of oxidized-LDL-induced inflammation, and protection of endothelial cells from oxidized-LDL-induced apoptosis, were all significantly improved by H2 treatment. In addition, H2 treatment increased the effective rate in down-regulating plasma levels of total cholesterol (47.06% vs 17.65%) and LDL cholesterol (47.06% vs 23.53%). Western blot analysis revealed a marked decrease in apolipoprotein B100 and an increase in apolipoprotein M in plasma of the H2 group. Finally H2 treatment resulted in a significant reduction in the levels of several inflammatory and oxidative stress indicators in whole plasma and HDL particles.
CONCLUSIONS
H2 activates ATP-binding cassette transporter A1-dependent efflux, enhances HDL antiatherosclerotic functions, and has beneficial lipid-lowering effects. The present findings highlight the potential role of H2 in the regression of hypercholesterolemia and atherosclerosis.