Characterization of two novel human small heat shock proteins:: protein kinase-related HspB8 and testis-specific HspB9
Characterization of two novel human small heat shock proteins:: protein kinase-related HspB8 and testis-specific HspB9
复制标题
DOI：
10.1016/s0167-4781(01)00237-8
复制
发表时间：
2001-07-30
期刊：
影响因子：
--
通讯作者：
De Jong, WW
中科院分区：
文献类型：
--
作者：
Kappé, G;Verschuure, P;De Jong, WW
使用基于保守的α-晶体蛋白结构域的搜索图谱，我们追踪到了两个新的人类sHsps。其中一个是已知的第八个人类SHSP，因此命名为HspB8，最近被描述为丝氨酸-苏氨酸蛋白激酶(H11)，但未被鉴定为SHSP(C.C.Smith，Y.X.Yu，M.Kulka，L.Aurelian，J.Biol)。化学。275(2000))。Northern blotting表明，HspB8/H11和大多数其他sHsps一样，主要在骨骼肌和心脏中转录。另一种命名为HspB9，在睾丸中特异表达，尤其是在粗线期晚期至伸长期的生精细胞中表达。哺乳动物的sHsps通常高度保守，而小鼠的HspB9与人的HspB9有38%的序列差异，这可能证实了它的性别相关作用。(C)2001 Elsevier Science B.V.保留所有权利。
Using search profiles based on the conserved alpha -crystallin domain that is characteristic for small heat shock proteins (sHsps), we traced two new human sHsps. One of these, being the eighth known human sHsp and thus named HspB8, was recently described as a serine-threonine protein kinase (H11), but not identified as an sHsp (C.C. Smith, Y.X. Yu, M. Kulka, L. Aurelian, J. Biol. Chem. 275 (2000)). Northern blotting showed that HspB8/H11 is predominantly transcribed in skeletal muscle and heart, like most other sHsps. The other, named HspB9, is specifically expressed in testis, notably in the spermatogenic cells from late pachytene spermatocyte stage till elongate spermatid stage. While mammalian sHsps are generally highly conserved, mouse HspB9 shows 38% sequence difference with human HspB9, which may confirm its sex-related role. (C) 2001 Elsevier Science B.V. All rights reserved.