Comparative proteomic analysis of the shoot apical meristem in maize between a ZmCCT-associated near-isogenic line and its recurrent parent.
Comparative proteomic analysis of the shoot apical meristem in maize between a ZmCCT-associated near-isogenic line and its recurrent parent.
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ZmCCT 相关近等基因系及其轮回亲本之间玉米茎尖分生组织的比较蛋白质组学分析
DOI：
10.1038/srep30641
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发表时间：
2016-07-29
影响因子：
4.6
通讯作者：
Chen Y
中科院分区：
文献类型：
--
作者：
Wu L;Wang X;Wang S;Wu L;Tian L;Tian Z;Liu P;Chen Y
ZmCCT是影响光周期反应的重要基因之一，在长日照条件下延迟玉米(Zea Mays)的开花。在这项研究中，我们使用基于相对和绝对量化等压标签(ITRAQ)技术的蛋白质组学方法来鉴定近等基因系(NIL)与其轮回亲本之间的差异表达蛋白质，对比ZmCCT的等位基因。共鉴定出5,259种不同的蛋白质。其中，NIL-CML系(ZmCCT阳性)和H4系(ZmCCT阴性)差异表达的蛋白质有386个。功能分类表明，差异蛋白质主要参与能量产生、光合作用、信号转导、细胞组织和生物发生。结果表明，在顶端分生组织发育过程中，ZmCCT阳性系的细胞分裂蛋白、碳水化合物代谢相关蛋白和花抑制相关蛋白含量均高于ZmCCT阴性系。这些结果结合形态观察表明，ZmCCT对开花的影响可能是通过影响这些生物学过程中的一个或全部而引起的。虽然这些假定的相关蛋白的确切作用仍有待研究，但我们利用蛋白质组学方法获得的结果有助于更好地理解玉米植株的光周期机制。
The ZmCCT, one of the most important genes affecting photoperiod response, delays flowering under long-day conditions in maize (Zea mays). In this study we used the isobaric tags for relative and absolute quantification (iTRAQ) technique-based proteomics approach to identify differentially expressed proteins between a near-isogenic line (NIL) and its recurrent parent, contrasting in alleles of ZmCCT. A total of 5,259 distinct proteins were identified. Among them, 386 proteins were differentially expressed between NIL-cml line (ZmCCT-positive) and H4 line (ZmCCT-negative). Functional categorization showed that the differentially proteins were mainly involved in energy production, photosynthesis, signal transduction, and cell organization and biogenesis. Our results showed that during shoot apical meristem (SAM) development cell division proteins, carbohydrate metabolism–related proteins, and flower inhibition-related proteins were more abundant in the ZmCCT-positive line than the ZmCCT-negative line. These results, taken together with morphological observations, showed that the effect of ZmCCT on flowering might be caused by its effect on one or all of these biological processes. Although the exact roles of these putative related proteins remain to be examined, our results obtained using the proteomics approach lead to a better understanding of the photoperiodicity mechanism in maize plants.