Levels of ovalbumin messenger RNA sequences in nonoviduct tissues of the chicken.
Levels of ovalbumin messenger RNA sequences in nonoviduct tissues of the chicken.
复制标题
鸡非输卵管组织中卵清蛋白信使 RNA 序列的水平。
DOI：
10.1016/0012-1606(80)90180-3
复制
发表时间：
1980
影响因子：
2.7
通讯作者：
M. Getz
中科院分区：
文献类型：
--
作者：
T. Ono;M. Getz
采用RNA-cDNA杂交技术，检测了鸡非输卵管组织中卵清蛋白mRNA在鸡肝、脾、雌激素刺激的鸡肝和10日龄鸡胚的核质组分中的表达水平。一般来说，研究的组织显示很少或无法检测到卵清蛋白RNA，检测限约为总RNA的10 - 5%。结果表明，卵清蛋白基因是转录不活跃或转录只有非常罕见的非输卵管组织，和不同的靶组织的反应，肝脏和输卵管雌激素刺激主要驻留在转录的控制。
The levels of ovalbumin mRNA sequence in nonoviduct tissues of the chicken were examined in nuclear and cytoplasmic fractions of hen liver, spleen, estrogen-stimulated chick liver, and 10-day-old embryo using RNA-cDNA hybridization. In general, the tissues studied revealed little or no detectable ovalbumin RNA, at the limit of detection of some 10−5% of total RNA. The results suggest that the ovalbumin gene is either transcriptionally inactive or transcribed only very infrequently in nonoviduct tissues, and that the different target tissue responses of liver and oviduct to estrogen stimulation resides largely in the control of transcription.