Entire industrial chain botanical origin authenticity control of ginseng formula granule products using simple PCR-based identification
Entire industrial chain botanical origin authenticity control of ginseng formula granule products using simple PCR-based identification
复制标题
基于PCR的简单鉴定对人参配方颗粒产品进行全产业链植物来源真伪控制
DOI：
10.1016/j.indcrop.2018.07.032
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发表时间：
2018-11-01
影响因子：
5.9
通讯作者：
Yuan，Yuan
中科院分区：
文献类型：
--
作者：
Jiang，Chao;Liu，Juan;Yuan，Yuan
人参配方颗粒是从人参的根中提取的。人参，是最重要的中国工业草药产品之一。区分 P.人参及其密切相关的替代品，P。西洋参（西洋参）和 P.配方颗粒中的三七（Notoginseng）是人参产业链中的关键问题。产业链始于人参作物，经过一系列草药制备程序——加工、提取、浓缩、干燥和制粒。在这些过程中，形态外观和微观特征被破坏，并且植物化学特征因提取物操作、配制过程和储存条件而变化很大。因此，在整个工业配方颗粒生产过程的关键阶段，有必要采用统一的真实性控制方法。本研究建立了一种简单的等位基因特异性鉴定方法，用于人参药物、提取物和配方颗粒的统一植物来源真实性控制。人参特异性引物和非人参特异性引物。采用来自高拷贝数 18S rDNA 的人参特异性引物进行人参真伪鉴定。它们用于从原植物、生药、提取物和配方颗粒中生成166bp的片段。该方法不仅能够区分P。相关物种的人参，还可以检测人参产品中的掺假物。本研究应用统一的DNA标记和技术在整个人参颗粒产业链中进行质量控制。
Ginseng formula granule is derived from the root ofP. ginseng, and is one of the most important Chinese industrial herbal products. Discriminating betweenP. ginsengand its closely-related substitutes,P. quinquefolius(American ginseng) andP. notoginseng(Notoginseng) in formula granule is a critical issue in the ginseng industrial chain. The industrial chain begins with the ginseng crop, which undergoes a series of herbal preparation procedures—processing, extraction, concentration, desiccation, and granulation. During these processes, morphological appearances and microscopic characteristics are destroyed, and phytochemical profiles vary greatly by extract manipulation, formulation process, and storage conditions. Therefore, a uniform authenticity control method is necessary for the critical stages in the entire industrial formula granule production process. In this study, a simple allele-specific identification method was established for uniform botanical origin authenticity control of ginseng drugs, extracts, and formula granule.P. ginseng-specific primers and non-P. ginseng–specific primers from high copy number 18S rDNA were employed for ginseng authenticity identification. They were used to generate 166 bp fragments from the original plant, crude drug, extract, and formula granule. The method was capable not only of distinguishingP. ginsengfrom related species, but also of detecting adulterants in ginseng products. This study applies a uniform DNA marker and techniques for quality control in the entire ginseng granule industrial chain.