Analysis of rat repetitive DNA sequences.
Analysis of rat repetitive DNA sequences.
复制标题
大鼠重复 DNA 序列的分析。
DOI：
10.1021/bi00594a008
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发表时间：
1978
期刊：
影响因子：
2.9
通讯作者：
J. Bonner
中科院分区：
文献类型：
--
作者：
W. Pearson;J. R. Wu;J. Bonner
重复序列组织的参数已经在大鼠基因组中被测量到。使用熔融、羟基磷灰石结合和单链特异性核酸酶消化的实验已经被用来测量重复DNA序列的数量、长度和排列。复性、融化或S1核酸酶消化1.0kbP的DNA片段显示，约有20%的大鼠DNA序列重复3000倍。从4.0kbP DNA片段复性的双链在核酸酶消化后显示出两个重复的大小片段。大约60%的重复序列的长度为0.2-0.4kbp，其余的长于1.5kbp。通过对带有重复序列的不同长度的DNA片段进行羟基磷灰石分级来测量重复序列的排列。重复的DNA序列散布在超过70%的大鼠基因组中的2.5kbp长的非重复序列中。在大鼠体内大约有350个不同的3000倍短重复序列，散布在60万个非重复DNA序列中。
Parameters of repetitive sequence organization have been measured in the rat genome. Experiments using melting, hydroxylapatite binding, and single strand specific nuclease digestion have been used to measure the number, length, and arrangement of repeated DNA sequences. Renaturation and melting or S1 nuclease digestion of 1.0 kbp DNA fragment show about 20% of rat DNA sequences are 3000-fold repeated. Renatured duplexes from 4.0 kbp DNA fragments display two repetitive size fractions after nuclease digestion. About 60% of the repeated sequences are 0.2-0.4 kbp long while the remainder are longer than 1.5 kbp. The arrangement of the repeated sequences has been measured by hydroxylapatite fractionation of DNA fragments of varying lengths bearing a repeated sequence. Repeated DNA sequences are interspersed among 2.5 kbp long nonrepeated sequences throughout more than 70% of the rat genome. There are approximately 350 different 3000-fold short repeated sequences in the rat interspersed among 600,000 nonrepeated DNA sequences.