Protective effects of ethanol extract from Portulaca oleracea L on dextran sulphate sodium-induced mice ulcerative colitis involving anti-inflammatory and antioxidant.
Protective effects of ethanol extract from Portulaca oleracea L on dextran sulphate sodium-induced mice ulcerative colitis involving anti-inflammatory and antioxidant.
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发表时间：
2016-05
影响因子：
2.2
通讯作者：
Xiao-hong Yang;Yongmei Yan;Jian-kang Li;Zhishu Tang;Jing Sun;Huan Zhang;S. Hao;A. Wen;Li Liu
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作者：
Xiao-hong Yang;Yongmei Yan;Jian-kang Li;Zhishu Tang;Jing Sun;Huan Zhang;S. Hao;A. Wen;Li Liu
马齿苋是一种常用的药食两用草本植物，在世界许多国家都有分布。许多研究表明，POL具有广泛的药理作用，如抗炎、肝病等。在临床研究中，POL多用于治疗UC，临床疗效较好，但其作用机制和科学内涵尚不清楚。本研究从氧化应激和炎症途径两个方面研究了蒲公英乙醇提取物对葡聚糖硫酸钠诱导的C57BL/6小鼠UC的保护作用。结果表明，蒲公英乙醇提取物可通过增加结肠长度、减轻体重减轻和疾病活动指数评分，通过丙二醛、一氧化氮、超氧化物歧化酶活性抑制氧化应激反应，减少促炎细胞因子(肿瘤坏死因子α、白介素1β和白介素6)的基因表达以及肿瘤坏死因子α和核因子kB p65的蛋白表达，从而对DSS所致UC具有有效的保护作用。这些结果可能证明POL可能是一种从食用植物中提取的有用而有效的植物化合物，通过其氧化应激和炎症活性用于治疗UC。
Portulaca oleracea L., (POL) is one of commonly used medicine-food herbs and has a cosmopolitan distribution in many countries. Many studies showed that POL exhibited a wide range of pharmacological effects such as anti-inflammatory and liver complaints. In the clinical studies, POL was usually used for the treatment of UC disease and the clinical efficacy was well, but the mechanism and scientific intension was still unknown. In the present study, we studied the protective effects of the ethanol extract from POL on dextran sulphate sodium-induced UC in C57BL/6 mice model through oxidative stress and inflammatory pathway. The results demonstrated that the ethanol extract from POL could exhibit the effective protection for the DSS induced UC by increasing the colon length, decreasing body weight loss and the disease activity index score, inhibiting oxidative stress response through the MDA, NO, SOD activities, reducing the mRNA expressions of pro-inflammatory cytokines (TNF-α, IL-1β and IL-6) and the protein expressions of TNF-α and NF-kB p65. These results may prove that POL could be considered as a useful and effective botanical compound from the edible plant to be used in UC through the oxidative stress and inflammatory activities.