Nanoscale fluorescent metal-organic framework composites as a logic platform for potential diagnosis of asthma
Nanoscale fluorescent metal-organic framework composites as a logic platform for potential diagnosis of asthma
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纳米级荧光金属有机框架复合材料作为哮喘潜在诊断的逻辑平台
DOI：
10.1016/j.bios.2019.01.011
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发表时间：
2019
影响因子：
12.6
通讯作者：
Qian Guodong
中科院分区：
文献类型：
--
作者：
Zhang Xin;Fang Liquan;Jiang Ke;He Huajun;Yang Yu;Cui Yuanjing;Li Bin;Qian Guodong
哮喘是一种常见的慢性疾病，肺内硫化氢（H2S）产生的减少被认为是哮喘的早期检测生物标志物。然而，生物系统中H2S的检测仍然是一个挑战;因为它要求设计的传感器具有以下特征：纳米尺寸，良好的生物相容性，实时检测，高选择性/灵敏度和良好的水稳定性。在这里，我们提出了使用纳米级荧光金属有机框架（MOF）复合物Eu 3 +/Ag+@UiO-66-（COOH）2（以下简称为EAUC）作为一个逻辑平台，通过检测生物标志物H2S初步诊断哮喘的潜力。这种基于Eu 3 +@UiO-66-（COOH）2（EUC）的INHIBIT逻辑门可以通过选择Ag+和H2S作为输入并通过监测荧光信号（I615）作为输出来产生。我们的荧光研究表明，EAUC具有良好的选择性，极高的灵敏度（检测限：23.53 μM），和实时原位检测H2S。此外，在PC 12细胞中的MTT分析表明，EAUC具有低细胞毒性和良好的生物相容性，适合于在体内检测生物标志物H2S，如通过成功检测稀释的血清样品中的加标H2S所证明的。本研究为基于MOF的哮喘临床诊断提供了一种新的思路。
Asthma is a common chronic disorder, and the decreased hydrogen sulfide (H2S) production in the lung has been considered as an early detection biomarker for asthma. However, the detection of H2S in biological systems remains a challenge; because it requires the designed sensors to have the following features: nanoscale size, good biocompatibility, real-time detection, high selectivity/sensitivity, and good water stability. Here, we propose the potential of using nanoscale fluorescent metal–organic framework (MOF) composites Eu3+/Ag+@UiO-66-(COOH)2(hereafter denoted asEAUC) as a logic platform for tentative diagnosis of asthma by detecting the biomarker H2S. This INHIBIT logic gate based on Eu3+@UiO-66-(COOH)2(EUC)can be produced by choosing Ag+and H2S as inputs and by monitoring the fluorescent signal (I615) as an output. Our fluorescent studies indicate that theEAUCexhibits excellent selectivity, extreme sensitivity (limit of detection: 23.53 μM), and real-time in situ detection of H2S. Further, MTT analysis in PC12 cells shows that theEAUCpossesses low cytotoxicity and favourable biocompatibility that are suitable for the detection of biomarker H2S in vivo, as demonstrated by the successful detection of spiked H2S in the diluted serum samples. This work represents the possibility of using MOF-based logic platform for tentative diagnosis of asthma in clinical medicine.