Oxypurine and purine nucleoside concentrations in renal vein of allograft are potential markers of energy status of renal tissue
Oxypurine and purine nucleoside concentrations in renal vein of allograft are potential markers of energy status of renal tissue
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DOI：
10.1016/j.arcmed.2006.07.010
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发表时间：
2007-02-01
影响因子：
7.7
通讯作者：
Ciechanowski, Kazimierz
中科院分区：
文献类型：
--
作者：
Domanski, Leszek;Safranow, Krzysztof;Ciechanowski, Kazimierz
背景缺血再灌注损伤导致的器官功能损害是实体器官移植中的一个重要问题。由缺血诱导的细胞改变为再灌注阶段期间发生的后续损伤准备组织。嘌呤核苷和氧代嘌呤是腺嘌呤核苷酸降解的产物。再灌注和复氧过程中伴随着活性氧和自由基的产生，导致移植组织的损伤。本研究的目的是测量腺嘌呤核苷酸及其代谢产物的浓度在肾移植静脉，以及在受体的外周静脉在再灌注期间，并评估其作为组织代谢的标志物在肾移植物的有用性。该研究招募了20名肾移植受者。在移植肾血管与受体髂血管吻合之前，从受体尺静脉采集第一份血样。移植肾再灌注后5分钟，从移植肾和尺静脉取样，用红外摄像机测量。采用高效液相色谱法（HPLC）测定全血和血浆中三磷酸腺苷（ATP）、一磷酸腺苷（AMP）、鸟苷（Guo）、次黄嘌呤（Hyp）、黄嘌呤（Xan）、次黄嘌呤（Ino）、尿酸（UA）和尿苷（Urd）的浓度。移植肾再灌注后静脉血Hyp和Xan浓度明显高于再灌注前和再灌注后外周静脉血Hyp和Xan浓度。本研究的结果表明，肾静脉和外周静脉之间的Hyp和Xan浓度的差异反映了再灌注过程中肾组织的代谢变化，可能是有用的移植物功能监测再灌注期间。(c)2007年IMSS。爱思唯尔公司出版
Background. Impairment of organ function derived from ischemia-reperfusion injury is an important problem in solid organ transplantation. Cell alterations induced by ischemia prime the tissue for subsequent damage that occurs during the reperfusion phase. Purine nucleosides and oxypurines are products of adenine nucleotides degradation. Reperfusion and reoxygenation are accompanied by production of reactive oxygen species and free radicals, which lead to damage of graft tissue. The aim of this study was to measure concentrations of adenine nucleotides and their metabolites in renal allograft vein as well as in recipient's peripheral veins during the reperfusion period and to evaluate their usefulness as markers of tissue metabolism in kidney allografts.Methods. The study enrolled 20 renal transplant recipients. The first blood sample was taken from the recipient's ulnar vein before anastomosing of the kidney graft's vessels with recipient's iliac vessels. Samples were then taken from the renal allograft and ulnar veins 5 min after total graft reperfusion measured with an infrared camera. High-performance liquid chromatography (HPLC) was performed to measure whole blood and plasma concentrations of adenosine triphosphate (ATP), adenosine monophosphate (AMP), guanosine (Guo), inosine (Ino), hypoxanthine (Hyp), xanthine (Xan), uric acid (UA), and uridine (Urd).Results. Hyp and Xan concentrations were significantly increased in renal allograft vein after reperfusion as compared with peripheral vein during the pre- and post-reperfusion periods.Conclusions. The results of the present study suggest that differences in Hyp and Xan concentrations between renal and peripheral veins reflect metabolic alterations in renal tissue during reperfusion and may be useful for graft function monitoring during reperfusion. (c) 2007 IMSS. Published by Elsevier Inc.