Natively disordered proteins: functions and predictions.
Natively disordered proteins: functions and predictions.
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DOI：
10.2165/00822942-200403020-00005
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发表时间：
2004-01-01
期刊：
影响因子：
--
通讯作者：
Dunker, A Keith
中科院分区：
文献类型：
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作者：
Romero, Pedro;Obradovic, Zoran;Dunker, A Keith
蛋白质至少可以以三种形式存在：有序形式（固体状），部分折叠形式（塌陷，熔融球状或液体状）和扩展形式（扩展，随机线圈状或气体状）。蛋白质三位一体假说有两个组成部分：(i)给定的天然蛋白质可以是三种形式中的任何一种，取决于序列和环境；（ii）函数可以由三种形式中的任何一种或它们之间的转换产生。在这项研究中，生物信息学和数据挖掘被用于研究蛋白质的内在无序性，并开发基于神经网络的自然无序区（PONDR）预测器，该预测器可以区分有序和无序残基，准确率高达84%。对内在无序的预测表明，这三个王国遵循真细菌<古细菌的无序排序
Proteins can exist in at least three forms: the ordered form (solid-like), the partially folded form (collapsed, molten globule-like or liquid-like) and the extended form (extended, random coil-like or gas-like). The protein trinity hypothesis has two components: (i) a given native protein can be in any one of the three forms, depending on the sequence and the environment; and (ii) function can arise from any one of the three forms or from transitions between them. In this study, bioinformatics and data mining were used to investigate intrinsic disorder in proteins and develop neural network-based predictors of natural disordered regions (PONDR) that can discriminate between ordered and disordered residues with up to 84% accuracy. Predictions of intrinsic disorder indicate that the three kingdoms follow the disorder ranking eubacteria < archaebacteria