ENHANCED EXPRESSION OF EPIDERMAL GROWTH-FACTOR RECEPTOR CORRELATES WITH ALTERATIONS OF CHROMOSOME-7 IN HUMAN PANCREATIC-CANCER
ENHANCED EXPRESSION OF EPIDERMAL GROWTH-FACTOR RECEPTOR CORRELATES WITH ALTERATIONS OF CHROMOSOME-7 IN HUMAN PANCREATIC-CANCER
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DOI：
10.1073/pnas.83.14.5141
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发表时间：
1986-07-01
影响因子：
11.1
通讯作者：
TRENT, J
中科院分区：
文献类型：
--
作者：
KORC, M;MELTZER, P;TRENT, J
最近，表皮生长因子（EGF）受体基因被定位到7号染色体的短臂7p染色体上[Shimizu， N., Kondo， I., Gamou， M. A. Behzadian， A. and Shimizu， Y.（1984）体细胞分子基因，10,45-53]。利用EGF结合的饱和度研究、核核学和cDNA杂交实验，我们试图确定在培养的人胰腺癌细胞中，7号染色体的剂量或改变与EGF受体表达增强之间是否存在相关性。与125i标记的EGF在4度下进行饱和结合研究。四种已建立的人胰腺癌细胞系：T3M4、PANC-1、COLO 357和UACC-462。结合数据分析显示，所有四种细胞系中EGF受体的数量增加。染色体显带分析显示，细胞系T3M4 - PANC-1和COLO 357的7p染色体克隆性结构改变，而UACC-462显示7号染色体的多个拷贝。使用放射性标记的EGF受体cDNA探针进行的杂交研究未能在任何细胞系中证明DNA序列扩增，但证实这些细胞中EGF受体mRNA的存在与EGF受体数量大致成比例。我们的研究结果表明，EGF受体在人胰腺癌中的表达增强可能与7号染色体的结构或数量改变有关。
Recently, the gene for the epidermal growth factor (EGF)receptor has been mapped to chromosome 7p, the short arm of chromosome 7 [Shimizu, N., Kondo, I., Gamou, M. A. Behzadian, A. and Shimizu, Y. (1984) Somatic Cell Mol. Genet. 10, 45-53]. Utilizing EGF binding in saturation studies, karyology, and cDNA hybridization experiments, we have sought to determine whether there is a correlation between dosage or alteration of chromosome 7 and enhanced expression of EGF receptor in cultured human pancreatic carcinoma cells . Saturation binding studies with 125I-labeled EGF were performed at 4.degree. C with four established human pancreatic cancer cell lines: T3M4, PANC-1, COLO 357, and UACC-462. Analysis of binding data revealed enhanced numbers of EGF receptors in all four cell lines. Chromosome banding analysis revealed clonal structural alterations of chromosome 7p in the cell lines T3M4 PANC-1, and COLO 357, whereas UACC-462 displayed multiple copies of chromosome 7. Hybridization studies using a radiolabeled EGF receptor cDNA probe failed to demonstrate DNA sequence amplification in any cell line but confirmed the presence of EGF receptor mRNA in these cells in approximate proportion to EGF receptor number. Our results suggest that enhanced expression of EGF receptor in human pancreatic cancer can be associated with either structural or numerical alterations of chromosome 7.