Clinical efficacy of spermatid conception: analysis using a new spermatid classification scheme.
Clinical efficacy of spermatid conception: analysis using a new spermatid classification scheme.
复制标题
精子细胞受孕的临床疗效：使用新的精子细胞分类方案进行分析。
DOI：
10.1093/humrep/14.5.1279
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发表时间：
1999
影响因子：
6.1
通讯作者：
J. Tesarik
中科院分区：
文献类型：
--
作者：
M. Sousa;A. Barros;K. Takahashi;C. Oliveira;J. Silva;J. Tesarik
50个圆形精子细胞注射(ROSI)和20个加长精子细胞注射(ELSI)治疗周期的受精和妊娠结局与周期的不同特征有关，特别是参照精子细胞发育阶段的分类方法进行评估。虽然这种分类包括八个阶段，但完整的传导阻滞大多是在最早的阶段(SA1)或最晚的阶段(SD1和SD2)发现的。其中SA1期50例(71%)，SD1期8例(11%)，SD2期10例(14%)。只有2例(3%)生精受阻于中期(SB2)。在全球范围内，ELSI的受精率高于ROSI。在ROSI周期中没有妊娠，而ELSI周期中有9例妊娠。其中两个(都有SD2精子细胞)在怀孕早期自然流产。在7个正在进行的妊娠中，5个是单胞胎(2个是SD1精子细胞，2个是SD2精子细胞，1个是在注射SA2和SD1精子细胞后混合移植)，2个是双胞胎(1个是SD1精子细胞，另一个是SD2精子细胞)。使用SB2精子细胞的两个周期均未妊娠。这两个双胞胎中的一个已经生下了两个健康的孩子。
Fertilization and pregnancy outcomes of 50 round spermatid injection (ROSI) and 20 elongated spermatid injection (ELSI) treatment cycles are related to various characteristics of the cycles, with particular reference to spermatid developmental stage as assessed by using a classification scheme adapted to this purpose. Although this classification includes eight stages, a complete block was mostly detected at the earliest stage (Sa1) or at the latest stages (Sd1 and Sd2). Thus, spermiogenesis was blocked at Sa1 stage in 50 cases (71%), at Sd1 stage in eight cases (11%) and at Sd2 stage in 10 cases (14%). Only in two cases (3%) was spermiogenesis blocked at an intermediate stage (Sb2). Globally, fertilization rates were higher for ELSI than for ROSI. No pregnancy was achieved in the ROSI cycles, whereas nine pregnancies resulted from the ELSI cycles. Two of them (both with Sd2 spermatids) ended in a first trimester spontaneous abortion. Of the seven ongoing pregnancies, five are singleton (two with Sd1 spermatids, two with Sd2 spermatids, and one after a mixed transfer after injection of Sa2 and Sd1 spermatids) and two are twin (one with Sd1 and the other with Sd2 spermatids). No pregnancy was achieved in the two cycles with Sb2 spermatids. One of the two twin pregnancies has already resulted in the birth of two healthy children.