Overexpression of Brassica campestris BcICE1 gene increases abiotic stress tolerance in tobacco.
Overexpression of Brassica campestris BcICE1 gene increases abiotic stress tolerance in tobacco.
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DOI：
10.1016/j.plaphy.2018.09.039
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发表时间：
2018-11
期刊：
影响因子：
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通讯作者：
Tengguo Zhang;J. Mo;Ke Zhou;Yan-Li Chang;Zigang Liu
中科院分区：
文献类型：
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作者：
Tengguo Zhang;J. Mo;Ke Zhou;Yan-Li Chang;Zigang Liu
本研究从白菜型油菜“龙优6号”中克隆了一个诱导CBF表达的基因（initiator of CBF expression 1，ICE 1），命名为BcICE 1。该基因编码499个氨基酸，含有一个典型的bHLH结构域，与拟南芥AtICE 1同源性为85.9%，BcICE 1定位于细胞核。低温胁迫下，转BcICE 1基因烟草的SOD、CAT、POD和APX活性以及SOD、CAT和POD基因的转录水平均高于野生型烟草。与野生型烟草相比，转BcICE 1基因烟草的脯氨酸、可溶性糖和叶绿素含量增加，而丙二醛和相对电导率降低。BcICE 1在烟草中的过表达增加了CBF 1、CBF 2和其他胁迫相关基因的表达。此外，在盐和PEG（25%）胁迫下，转BcICE 1基因烟草的APX和GPX活性以及可溶性糖和叶绿素含量均高于WT烟草。我们的研究结果表明BcICE 1在植物对非生物胁迫的响应中起着重要的作用。
In this study, a cDNA ofICE1(inducer of CBF expression 1) gene, namedBcICE1, was isolated from Brassica campestris ‘Longyou 6’. The deduced protein has 499 amino acids containing a typical bHLH domain and is highly identical with AtICE1 (85.9%) from Arabidopsis thaliana.BcICE1is located in the nucleus. The activities of SOD, CAT, POD, and APX and the transcriptional levels ofSOD,CAT, andPODgenes were higher in BcICE1-transgenic tobacco than in wild-type (WT) tobacco under cold stress. Compared with WT tobacco, proline, soluble sugar, and chlorophyll were enhanced, whereas malondialdehyde and relative conductivity were decreased in BcICE1-transgenic tobacco. The overexpression ofBcICE1in tobacco increased the expression ofCBF1,CBF2, and other stress-related genes. Moreover, under salt and PEG (25%) stress, the activities of APX and GPX and content of soluble sugar and chlorophyll in BcICE1-transgenic tobacco were higher than those in WT tobacco. Our results suggest thatBcICE1plays an important role in the response to abiotic stress.