The Asian arowana (Scleropages formosus) genome provides new insights into the evolution of an early lineage of teleosts.
The Asian arowana (Scleropages formosus) genome provides new insights into the evolution of an early lineage of teleosts.
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DOI：
10.1038/srep24501
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发表时间：
2016-04-19
影响因子：
4.6
通讯作者：
Shi Q
中科院分区：
文献类型：
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作者：
Bian C;Hu Y;Ravi V;Kuznetsova IS;Shen X;Mu X;Sun Y;You X;Li J;Li X;Qiu Y;Tay BH;Thevasagayam NM;Komissarov AS;Trifonov V;Kabilov M;Tupikin A;Luo J;Liu Y;Song H;Liu C;Wang X;Gu D;Yang Y;Li W;Polgar G;Fan G;Zeng P;Zhang H;Xiong Z;Tang Z;Peng C;Ruan Z;Yu H;Chen J;Fan M;Huang Y;Wang M;Zhao X;Hu G;Yang H;Wang J;Wang J;Xu X;Song L;Xu G;Xu P;Xu J;O'Brien SJ;Orbán L;Venkatesh B;Shi Q
亚洲龙鱼是世界上最昂贵的观赏鱼之一，也是濒危物种。它代表了硬骨鱼类的一个古老谱系：骨舌形目。在这里，我们提供了一个高质量的染色体水平参考基因组的女性黄金品种龙鱼使用深度鸟枪测序和高分辨率的连锁图谱相结合。此外，我们还为红色和绿色品种生成了两个基因组组装草图。系统基因组学分析支持骨舌类（骨舌）和Elopomorpha（鳗鱼和亲戚）之间的姐妹群关系，这两个分支一起形成了包括所有剩余硬骨鱼的姐妹群。龙鱼的基因组保留了完整的八个Hox簇，不像非洲蝴蝶鱼（Pantodon buchholzi），另一种骨舌鱼，只有五个Hox簇。三个品种之间的差异基因表达提供了深入了解颜色变异的遗传基础。在雌性龙鱼核型中发现了一条潜在的异配子性染色体，表明性别是由ZW/ZZ性染色体系统决定的。金龙鱼的高质量参考基因组和红色和绿色品种的草案汇编是了解亚洲龙鱼的生物学，适应和行为的宝贵资源。
The Asian arowana (Scleropages formosus), one of the world’s most expensive cultivated ornamental fishes, is an endangered species. It represents an ancient lineage of teleosts: the Osteoglossomorpha. Here, we provide a high-quality chromosome-level reference genome of a female golden-variety arowana using a combination of deep shotgun sequencing and high-resolution linkage mapping. In addition, we have also generated two draft genome assemblies for the red and green varieties. Phylogenomic analysis supports a sister group relationship between Osteoglossomorpha (bonytongues) and Elopomorpha (eels and relatives), with the two clades together forming a sister group of Clupeocephala which includes all the remaining teleosts. The arowana genome retains the full complement of eight Hox clusters unlike the African butterfly fish (Pantodon buchholzi), another bonytongue fish, which possess only five Hox clusters. Differential gene expression among three varieties provides insights into the genetic basis of colour variation. A potential heterogametic sex chromosome is identified in the female arowana karyotype, suggesting that the sex is determined by a ZW/ZZ sex chromosomal system. The high-quality reference genome of the golden arowana and the draft assemblies of the red and green varieties are valuable resources for understanding the biology, adaptation and behaviour of Asian arowanas.