Endomembrane proteomics reveals putative enzymes involved in cell wall metabolism in wheat grain outer layers.
Endomembrane proteomics reveals putative enzymes involved in cell wall metabolism in wheat grain outer layers.
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DOI：
10.1093/jxb/erv075
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发表时间：
2015-05
影响因子：
6.9
通讯作者：
Larré C
中科院分区：
文献类型：
--
作者：
Chateigner-Boutin AL;Suliman M;Bouchet B;Alvarado C;Lollier V;Rogniaux H;Guillon F;Larré C
小麦籽粒外层由几个专门的层组成，对发展、保护和最终用途具有决定性作用。细胞壁的异质性被强调，并与细胞壁机制组成的可能差异相关。谷物外层对种子发育起着提供能量、提供保护等重要作用。在食品工业中，被称为“麸皮”的谷物外层很有价值，因为它富含膳食纤维和其他有益营养。外层由细胞壁材料含量高的几个组织组成。在细胞壁合成活跃阶段，用特异性探针研究了颗粒外周组织的细胞壁组成。在细胞类型之间显示出相当大的细胞壁多样性。为了确定参与细胞壁合成的细胞机制，亚细胞蛋白质组学方法被用于针对大多数细胞壁多糖合成的高尔基体。将组织解剖为外果皮和中间层，分别鉴定出822和1304个蛋白。许多碳水化合物活性酶被发现：一些在两个外围颗粒部分，其他人只在一个组织。某些蛋白质家族对某一组分具有特异性，并且在其他物种中具有特征同源物，可能与特定细胞层中多糖的特异性检测有关。本报告提供了迄今为止研究较少的粮食细胞壁及其在有价值的外组织中的生物合成的新信息。更好地了解控制细胞壁组成的机制可以帮助改善谷物产品的一些品质性状（例如膳食纤维含量，生物质转化为生物燃料）。
Wheat grain outer layers, decisive for development, protection and end-uses, comprise several specialized layers. Cell wall heterogeneity is highlighted and correlated to probable differences in composition of wall machineries. Cereal grain outer layers fulfil essential functions for the developing seed such as supplying energy and providing protection. In the food industry, the grain outer layers called ‘the bran’ is valuable since it is rich in dietary fibre and other beneficial nutriments. The outer layers comprise several tissues with a high content in cell wall material. The cell wall composition of the grain peripheral tissues was investigated with specific probes at a stage of active cell wall synthesis. Considerable wall diversity between cell types was revealed. To identify the cellular machinery involved in cell wall synthesis, a subcellular proteomic approach was used targeting the Golgi apparatus where most cell wall polysaccharides are synthesized. The tissues were dissected into outer pericarp and intermediate layers where 822 and 1304 proteins were identified respectively. Many carbohydrate-active enzymes were revealed: some in the two peripheral grain fractions, others only in one tissue. Several protein families specific to one fraction and with characterized homologs in other species might be related to the specific detection of a polysaccharide in a particular cell layer. This report provides new information on grain cell walls and its biosynthesis in the valuable outer tissues, which are poorly studied so far. A better understanding of the mechanisms controlling cell wall composition could help to improve several quality traits of cereal products (e.g. dietary fibre content, biomass conversion to biofuel).