Genomic analysis of two Chinese strains of porcine reproductive and respiratory syndrome viruses with different virulence
Genomic analysis of two Chinese strains of porcine reproductive and respiratory syndrome viruses with different virulence
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DOI：
10.1007/s11262-010-0453-z
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发表时间：
2010-06-01
期刊：
影响因子：
1.6
通讯作者：
Cao, Yongchang
中科院分区：
文献类型：
--
作者：
Li, Yan;Xue, Chunyi;Cao, Yongchang
从猪繁殖与呼吸综合征病毒（PRRSV）中分离到两株，分别命名为GDQJ和GDBY1。试验接种结果表明，GDBY1致死率为100%，致死率为67%，GDQJ致死率为100%，无死亡。对全长基因组进行测序。同源性和系统发育分析表明，这两株病毒与中国高致病性PRRSV病毒亲缘关系密切。令人惊讶的是，在被认为是高毒力标记的nsp2编码区，同样有30个氨基酸（aa）缺失，在低毒力菌株GDQJ中存在。进一步对GDQJ基因组与中国高致病性PRRSV毒株进行综合分析发现，GDQJ基因组存在多种变异，分布在5′UTR、NSP1b、NSP2、NSP3、NSP5、NSP7、NSP9、NSP10、GP5和N区。本文的数据证实，仅nsp2编码区30aa缺失并不能作为中国出现的PRRSV高毒力毒株的可靠基因组指标。GDQJ菌株的基因组变异为进一步研究prrsv毒力决定因素提供了基础。
Two strains of porcine reproductive and respiratory syndrome virus (PRRSV) were isolated, designated GDQJ and GDBY1. Experimental inoculation showed that GDBY1, caused 100% morbidity and 67% mortality, while GDQJ, caused 100% morbidity but no death. Full-length genomes were sequenced. Homologic and phylogenetic analyses indicated that these two strains were closely related to Chinese highly pathogenic PRRSV strains. Surprisingly, identical 30 amino acids (aa) deletion in the NSP2-coding region, a presumed high virulence marker, was present in low virulent strain GDQJ. Further comprehensive analysis of GDQJ genome in comparison with Chinese highly pathogenic PRRSV strains revealed multiple genomic variations, distributing in 5' UTR, NSP1b, NSP2, NSP3, NSP5, NSP7, NSP9, NSP10, GP5, and N regions. Data present in this article confirm that the 30 aa deletion in the NSP2-coding region alone is not a reliable genomic indicator for the high virulence of PRRSV strains emerged in China. The genomic variations of GDQJ strain provided the basis for further studies of virulence determinants for PRRSVs.