Aversive odorant causing appetite decrease downregulates tyrosine decarboxylase gene expression in the olfactory receptor neuron of the blowfly, Phormia regina
Aversive odorant causing appetite decrease downregulates tyrosine decarboxylase gene expression in the olfactory receptor neuron of the blowfly, Phormia regina
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DOI：
10.1007/s00114-011-0865-1
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发表时间：
2011
影响因子：
--
通讯作者：
Y. Ishida;M. Ozaki
中科院分区：
文献类型：
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作者：
Y. Ishida;M. Ozaki
对于绿头蝇，在进食期间接触 d-柠檬烯 5 天会抑制长鼻伸展反射行为，因为大脑中的酪胺 (TA) 滴度会降低。 TA 由酪氨酸脱羧酶 (Tdc) 合成，并由 TA β-羟化酶 (Tbh) 催化生成章鱼胺 (OA)。为了解决绿头蝇 TA 滴度调节的机制，我们从 P. 克隆了 Tdc 和 Tbh cDNA。里贾纳（PregTdc 和 PregTbh）。推导的两种蛋白的氨基酸序列与果蝇相应蛋白在氨基酸水平上具有较高的一致性。PregTdc在触角、唇瓣和跗节中表达，PregTbh在头部表达，表明TA主要在感觉器官中合成，而OA主要在大脑中合成。 d-柠檬烯暴露显着降低了触角中的 PregTdc 表达，但在唇瓣和跗骨中没有显着降低，表明触角中表达的 PregTdc 是导致 TA 滴度降低的原因。 PregTdc 样免疫反应物质位于薄壁感器中。相反，OA/TA 受体 (PregOAR/TAR) 定位于厚壁感器。结果表明，d-柠檬烯抑制薄壁感觉器嗅觉受体神经元中 PregTdc 的表达，可能导致触角受体神经元 TA 水平降低。 TA可能通过投射神经元从受体神经元转移到大脑触角叶中的特定突触连接处，并发挥将厌恶气味信息传递到投射神经元和局部神经元的作用。
In the blowflyPhormia regina, exposure to d-limonene for 5 days during feeding inhibits proboscis extension reflex behavior due to decreasing tyramine (TA) titer in the brain. TA is synthesized by tyrosine decarboxylase (Tdc) and catalyzed into octopamine (OA) by TA ß-hydroxylase (Tbh). To address the mechanisms of TA titer regulation in the blowfly, we cloned Tdc and Tbh cDNAs fromP. regina(PregTdc and PregTbh). The deduced amino acid sequences of both proteins showed high identity to those of the corresponding proteins fromDrosophila melanogasterat the amino acid level.PregTdcwas expressed in the antenna, labellum, and tarsus whereasPregTbhwas expressed in the head, indicating that TA is mainly synthesized in the sensory organs whereas OA is primarily synthesized in the brain. d-Limonene exposure significantly decreasedPregTdcexpression in the antenna but not in the labellum and the tarsus, indicating that PregTdc expressed in the antenna is responsible for decreasing TA titer. PregTdc-like immunoreactive material was localized in the thin-walled sensillum. In contrast, the OA/TA receptor (PregOAR/TAR) was localized to the thick-walled sensillum. The results indicated that d-limonene inhibitsPregTdcexpression in the olfactory receptor neurons in the thin-walled sensilla, likely resulting in reduced TA levels in the receptor neurons in the antenna. TA may be transferred from the receptor neuron to the specific synaptic junction in the antennal lobe of the brain through the projection neurons and play a role in conveying the aversive odorant information to the projection and local neurons.