Immunogenicity and Protective Efficacy of Probiotics with EtIMP1C against Eimeria tenella Challenge
Immunogenicity and Protective Efficacy of Probiotics with EtIMP1C against Eimeria tenella Challenge
复制标题
益生菌与 EtIMP1C 对抗柔嫩艾美耳球虫挑战的免疫原性和保护功效
DOI：
10.29261/pakvetj/2021.009
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发表时间：
2021-01-01
影响因子：
2.3
通讯作者：
Yin, Guangwen
中科院分区：
文献类型：
--
作者：
Mohsin, Muhammad;Li, Lina;Yin, Guangwen
鸡球虫病是一种严重危害肉鸡的地方性疾病，给世界养鸡业造成了巨大的损失。疫苗在控制传染病方面通常是有效的;如在使用柔嫩艾美耳球虫攻击的本实验中所评估的，亚单位疫苗的有效性有望通过同时使用益生菌来改善。免疫映射蛋白1（IMP 1）是一种新的顶复门（Apicomplexans）的免疫原性蛋白，包括柔嫩艾美耳球虫（Eimeria tenella）。基于各种参数，如肠道病变、卵囊评分、饲料转化率和器官重量，评价益生菌和EtIMP 1C（柔嫩艾美耳球虫免疫映射蛋白1的一部分）的累积效应的抗球虫性能。还分析了免疫学和血液学参数的数据。与用FCA乳化的EtIMP 1C组相比，EtIMP 1C和益生菌给药组显示出较少的肠道病变、减少的卵囊脱落、令人满意的饲料转化率（FCR）和改善的血液学参数。两组间病变评分、卵囊评分、免疫学和血液学指标差异均无统计学意义（P>0.05）。实验工作表明，益生菌可能是一个很好的希望，因为它的抗氧化剂，免疫调节和促进生长的作用，对家禽球虫病单独或与疫苗，包括IMP 1C为基础的疫苗组合，但需要进一步的研究，以制定其剂量与疫苗以及不同菌株的益生菌对球虫病的影响。(C)2021年PVJ。版权所有
Coccidiosis is an endemic problem in broilers inflicting disastrous losses to worldwide poultry industry. Vaccines are generally effective in controlling the infectious diseases; the subunit vaccines effectiveness can hopefully be improved with concurrent use of probiotics as assessed in this experiment using an Eimeria tenella challenge. Immune mapped protein-1 (IMP1) is a novel immunogenic protein of Apicomplexans, including in Eimeria tenella. Anticoccidial performance of the cumulative effect of probiotics and EtIMP1C (Part of Eimeria tenella immune mapped protein1) was evaluated based on various parameters such as an intestinal lesion, oocyst scores, feed conversion ratio, and organ weight. Data were also analyzed on both immunological and hematological parameters. The EtIMP1C and probiotics administered group showed less intestinal lesion, decreased oocyst shedding, satisfactory feed conversion ratio (FCR) and improved hematological parameters as compared to EtIMP1C emulsified with FCA group. However, there was no statistically significant difference between the two groups (P>0.05), in aspect of lesion and oocyst scores, as well as immunological and hematological parameters. The experimental work showed that probiotics could be a good hope due to its antioxidant, immunomodulatory and growth promoting effect against poultry coccidiosis alone or in combination with vaccines, including IMP1C based vaccine, but further studies are required to formulate its dose with the vaccine as well as a different strain of probiotics effect against coccidiosis. (C) 2021 PVJ. All rights reserved