Nanoengineered Peptide-Grafted Hyperbranched Polymers for Killing of Bacteria Monitored in Real Time via Intrinsic Aggregation-Induced Emission
Nanoengineered Peptide-Grafted Hyperbranched Polymers for Killing of Bacteria Monitored in Real Time via Intrinsic Aggregation-Induced Emission
复制标题
纳米工程肽接枝超支化聚合物用于通过本征聚集诱导发射实时监测细菌杀灭
DOI：
10.1021/acsami.8b15921
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发表时间：
2018
影响因子：
9.5
通讯作者：
Wang Caiqi
中科院分区：
文献类型：
--
作者：
Zhao Jianliang;Dong Zhenzhen;Cui Hanrui;Jin Hongwei;Wang Caiqi
面对由耐药细菌引起的全球健康危机，抗菌肽及其类似物为这一普遍问题提供了令人兴奋的解决方案。在不引入荧光探针的情况下，构建了一种新型纳米工程肽接枝超支化聚合物（NPGHPs），该聚合物具有出色的抗菌活性和敏感的实时细菌检测。合成了具有聚集诱导发射效应的超支化聚酰胺胺（H-PAMAM）。然后，在H-PAMAM外围通过α-氨基酸n -羧基氢化物开环聚合制备NPGHPs。NPGHPs对多种细菌，特别是革兰氏阴性细菌具有高效的抗菌性能。在NPGHPs的AIE效应的基础上，探讨了NPGHPs与大肠杆菌的相互作用，NPGHPs的荧光强度依赖于e的数量。colipresent。因此，一种监测方法。建立了共聚焦法，检出限为1 × 104CFU mL-1。此外，NPGHPs用作荧光探针，通过点亮细菌来观察抗菌过程。NPGHPs可以穿透细菌膜，导致细胞破裂和凋亡。此外，NPGHPs对细菌的选择性优于哺乳动物细胞，具有广阔的临床应用前景。
Facing the global health crisis caused by drug-resistant bacteria, antimicrobial peptides and their analogues offer exciting solutions to this widespread problem. Without additionally introducing a fluorescent probe, novel nanoengineered peptide-grafted hyperbranched polymers (NPGHPs) are constructed for their combined outstanding antimicrobial activity and sensitive bacterial detection in real time. Hyperbranched polyamide amine (H-PAMAM) that exhibits aggregation-induced emission (AIE) effects is synthesized. Then, NPGHPs are prepared by ring-opening polymerization of α-amino acidN-carboxyanhydrides on the periphery of the H-PAMAM. The NPGHPs exhibit high-efficiency antibacterial properties against a wide spectrum of bacteria, especially against Gram-negative bacteria. On the basis of the AIE effect of NPGHPs, the interaction between NPGHPs andEscherichia coliis explored and the fluorescence intensity of NPGHPs is dependent on the number ofE. colipresent. Thus, a method for monitoringE. coliconcentration is developed, and the detection limit is 1 × 104CFU mL–1. Furthermore, NPGHPs are used as fluorescent probes to visualize antibacterial process via lighting-up bacteria. NPGHPs can penetrate the membrane of bacteria and cause cell rupture and apoptosis. In addition, the excellent selectivity of NPGHPs toward bacteria over mammalian cells makes them bright prospects for clinical applications.