Diversity of Bacillus thuringiensis Isolated from Western Ghats of Tamil Nadu State, India
Diversity of Bacillus thuringiensis Isolated from Western Ghats of Tamil Nadu State, India
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印度泰米尔纳德邦西高止山脉分离的苏云金芽孢杆菌多样性
DOI：
10.1007/s00284-009-9569-6
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发表时间：
2010
影响因子：
2.6
通讯作者：
V. Udayasuriyan
中科院分区：
文献类型：
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作者：
A. Ramalakshmi;V. Udayasuriyan
印度西高止山脉是世界十大“最热门的生物多样性热点地区”之一，它沿着印度西部，穿过泰米尔纳德邦等四个邦。西高止山脉唯一的生物多样性保护区是位于泰米尔纳德邦的尼尔吉里生物圈。在本研究中，从印度泰米尔纳德邦西高止山脉的所有 14 个不同分区采集了 525 个土壤样本。从 525 个土壤样品中分离出总共 316 个产生伴孢结晶内含物的苏云金芽孢杆菌 (Bt) 新分离株。在 316 个新的 Bt 分离株中观察到了 7 种不同类型的结晶内含物。与其他形状相比，26.9% 的 Bt 分离株主要存在立方体包涵体。通过 SDS-PAGE 对 316 个 Bt 分离株中的 70 个进行晶体蛋白谱的进一步表征，揭示了六种不同类型的晶体蛋白谱，即 135 和 65、135、95、65、43 和 30 kDa 晶体蛋白。从 Bt 分离物中纯化的晶体蛋白质量的变化揭示了印度泰米尔纳德邦西高止山脉中普遍存在的这种细菌的分子多样性。
The Western Ghats of India is the one of the world’s 10 “Hottest biodiversity hotspots” that runs along the western part of India through four states including Tamil Nadu. The only biodiversity reserve in the Western Ghats is the Nilgiri biosphere located in the Tamil Nadu state. In the present study, 525 soil samples were collected from all the 14 different divisions of the Western Ghats in Tamil Nadu state, India. A total of 316 new isolates of Bacillus thuringiensis (Bt) that produce parasporal crystalline inclusions were isolated from 525 soil samples. Seven different types of crystalline inclusions were observed in the 316 new isolates of Bt. Cuboidal inclusion was predominantly present in 26.9% of the Bt isolates when compared to other shapes. Further characterization of 70 of the 316 Bt isolates for crystal protein profile through SDS-PAGE revealed six different types of crystal protein profile viz., 135 and 65, 135, 95, 65, 43, and 30 kDa crystal proteins. Variation in the mass of crystal protein(s) purified from the isolates of Bt revealed molecular diversity of this bacterium prevalent in the Western Ghats of Tamil Nadu, India.
过氧化氢光解灭菌法对蜡样芽孢杆菌芽孢的杀芽作用
DOI：
--
发表时间：
2022
期刊：
影响因子：
--
作者：
宍戸駿一;白土翠;倉内美智子;中村圭祐;菅野太郎;庭野吉己
通讯作者：
庭野吉己