Borreliacidal activity of saliva of the tick Amblyomma americanum
Borreliacidal activity of saliva of the tick Amblyomma americanum
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DOI：
10.1111/j.0269-283x.2005.00546.x
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发表时间：
2005-03-01
影响因子：
1.9
通讯作者：
Piesman, J
中科院分区：
文献类型：
--
作者：
Ledin, KE;Zeidner, NS;Piesman, J
Amblyomma americanum（Linneaus）（阿卡里：硬蜱科）是人类和动物疾病的重要蜱媒介，不是莱姆病细菌病原体伯氏疏螺旋体的有效媒介，尽管其范围与莱姆病在美国的地理分布重叠。一种可能的机制，可以防止收购B。感染宿主产生伯氏螺旋体的主要原因是A. B上的美洲唾液。burgdorferi。本文提供的数据表明，在暴露于A. americanum唾液中，B显着较少，B。通过在暗视野显微镜下的螺旋体运动性和对死染色剂碘化丙啶的抗性来评估，与处理对照相比，伯氏螺旋体是活的。48小时后，低于13%的唾液接触B。伯多菲还活着。相比之下，明显更多的B。与肩突硬蜱（阿卡里：硬蜱科）唾液接触后24或48 h，伯氏硬蜱的存活率分别为100%和100%。americanum唾液或处理对照。
Amblyomma americanum (Linneaus) (Acari: Ixodidae), an important tick vector of human and animal disease, is not a competent vector of the bacterial agent of Lyme disease, Borrelia burgdorferi, although its range overlaps the geographical distribution of Lyme disease within the United States. A possible mechanism that could prevent acquisition of B. burgdorferi spirochetes from infected hosts is the toxic effect of A. americanum saliva on B. burgdorferi. The data presented here indicate that after 24 and 48 h of exposure to A. americanum saliva, significantly fewer B. burgdorferi were alive compared to treatment controls as assessed by spirochete motility under dark-field microscopy and resistance to the dead stain, propidium iodide. After 48 h, fewer than 13% of saliva-exposed B. burgdorferi were alive. In contrast, significantly more B. burgdorferi exposed to Ixodes scapularis (Acari: Ixodidae) saliva survived after 24 or 48 h compared to A. americanum saliva or treatment controls.