Beepath: An ordered quantitative-PCR array for exploring honey bee immunity and disease
Beepath: An ordered quantitative-PCR array for exploring honey bee immunity and disease
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DOI：
10.1016/j.jip.2006.04.004
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发表时间：
2006-10-01
影响因子：
3.4
通讯作者：
Evans, Jay D.
中科院分区：
文献类型：
--
作者：
Evans, Jay D.
蜜蜂和其他昆虫面临许多重要的寄生虫和病原体，它们已经进化出行为，形态，生理和免疫防御。为了帮助验证蜜蜂免疫基因候选者并确定它们对病原体的反应，开发了定量PCR阵列以平行测量48种蜜蜂和病原体基因的转录水平。结果表明，该阵列可以准确地测量宿主和病原体转录本丰度，为评估蜜蜂免疫背后的环境和遗传成分提供了一种新的工具。通过使用共同的平台和化学（例如，SYBR-绿色嵌入染料），定量PCR阵列，如所描述的一个应该在无脊椎动物病理学的各种用途。爱思唯尔公司出版
Honey bees and other insects face many important parasites and pathogens against which they have evolved behavioral, morphological, physiological, and immune-based defenses. To help validate honey bee immune-gene candidates and determine their responsiveness to pathogens., a quantitative-PCR array was developed to measure transcript levels for 48 honey bee and pathogen genes in parallel. It is shown that this array can accurately measure host and pathogen transcript abundance, providing a new tool for assessing the environmental and genetic components behind honey bee immunity. By using common platforms and chemistries (e.g., SYBR-Green intercalating dye), quantitative-PCR arrays such as the one described should have diverse uses in invertebrate pathology. Published by Elsevier Inc.