Combined effect of hydrogen peroxide induced oxidative stress and IL-1α on IL-8 production in CaCo-2 cells (a human colon carcinoma cell line) and normal intestinal epithelial cells
Combined effect of hydrogen peroxide induced oxidative stress and IL-1α on IL-8 production in CaCo-2 cells (a human colon carcinoma cell line) and normal intestinal epithelial cells
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DOI：
10.1023/a:1023813710941
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发表时间：
2003-06-01
期刊：
影响因子：
5.1
通讯作者：
Okabe, H
中科院分区：
文献类型：
--
作者：
Yamamoto, K;Kushima, R;Okabe, H
氧化应激、il -1 α和IL-8是引起胃肠道粘膜炎症的已知因素。我们检测了短暂暴露于过氧化氢诱导的CaCo-2细胞（一种人结肠癌细胞系）和人肠上皮细胞中IL-8的反应。此外，我们研究了暴露于氧化应激，然后是il -1 α，是否可以调节IL-8的产生。过氧化氢处理后，小鼠IL-8 mRNA表达瞬间上调。过氧化氢诱导的氧化应激也能促进IL-8的分泌。暴露于过氧化氢，然后是il -1 α，比单独使用il -1 α提高了IL-8的产生。因此，我们观察到氧化应激和il -1 α共同促进IL-8的产生。
Oxidative stress, IL-1alpha, and IL-8 are known to contribute to mucosal inflammation of the gastrointestinal tract. We examined the IL-8 response after brief exposure to hydrogen peroxide induced oxidative stress in CaCo-2 cells (a human colon carcinoma cell line) and in human intestinal epithelial cells. In addition, we examined whether exposure to oxidative stress, followed by IL-1alpha, could modulate IL-8 production. A transient up-regulation of IL-8 mRNA expression was observed after hydrogen peroxide treatment. Hydrogen peroxide induced oxidative stress was also observed to promote IL-8 secretion. Exposure to hydrogen peroxide, followed by IL-1alpha, enhanced IL-8 production over that achieved with IL-1alpha alone. Thus, oxidative stress and IL-1alpha were observed to cooperatively enhance IL-8 production.