Widespread ability of fungi to drive quinone redox cycling for biodegradation
Widespread ability of fungi to drive quinone redox cycling for biodegradation
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DOI：
10.1093/femsle/fnw105
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发表时间：
2016-06-01
影响因子：
2.1
通讯作者：
Schlosser, Dietmar
中科院分区：
文献类型：
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作者：
Krueger, Martin C.;Bergmann, Michael;Schlosser, Dietmar
木腐真菌具有显著不同的细胞外氧化机制，包括酶，如漆酶和过氧化物酶，和芬顿化学。先前已经报道了通过醌类的氧化还原循环生物驱动芬顿化学的能力存在于两个生态上不同的主要木腐担子菌群中。因此，我们调查了它是否在真菌生物中更普遍。对总共18种子囊菌和担子菌的多样性选择进行筛选以还原模型化合物2，6-二甲氧基苯醌，结果表明，所有研究的菌株都能够将其还原为相应的氢醌。在第二步中，解聚的合成聚合物聚苯乙烯磺酸盐被用作代理醌依赖性的基于Fenton的生物降解能力。一个不同的子集的菌株，包括环境中普遍存在的霉菌，白腐真菌，以及泥炭地和水生隔离物，引起大量的解聚指示醌氧化还原循环作为生物降解工具的有效就业。我们的研究结果也可能开辟新的途径，利用不同的真菌的生物修复的难降解有机污染物。
Wood-rotting fungi possess remarkably diverse extracellular oxidation mechanisms, including enzymes, such as laccase and peroxidases, and Fenton chemistry. The ability to biologically drive Fenton chemistry by the redox cycling of quinones has previously been reported to be present in both ecologically diverging main groups of wood-rotting basidiomycetes. Therefore, we investigated whether it is even more widespread among fungal organisms. Screening of a diverse selection of a total of 18 ascomycetes and basidiomycetes for reduction of the model compound 2,6-dimethoxy benzoquinone revealed that all investigated strains were capable of reducing it to its corresponding hydroquinone. In a second step, depolymerization of the synthetic polymer polystyrene sulfonate was used as a proxy for quinone-dependent Fenton-based biodegradation capabilities. A diverse subset of the strains, including environmentally ubiquitous molds, white-rot fungi, as well as peatland and aquatic isolates, caused substantial depolymerization indicative for the effective employment of quinone redox cycling as biodegradation tool. Our results may also open up new paths to utilize diverse fungi for the bioremediation of recalcitrant organic pollutants.