Critical role of dendritic cells in mouse mammary tumor virus in vivo infection
Critical role of dendritic cells in mouse mammary tumor virus in vivo infection
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DOI：
10.1128/jvi.02728-06
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发表时间：
2007-04-01
影响因子：
5.4
通讯作者：
Ross, Susan R.
中科院分区：
文献类型：
--
作者：
Cecilia Courreges, Maria;Burzyn, Dalia;Ross, Susan R.
小鼠乳腺肿瘤病毒(MMTV)是一种通过牛奶传播的贝塔病毒，可导致小鼠乳腺肿瘤。虽然乳腺上皮细胞是MMTV的最终目标，但该病毒利用宿主免疫系统的组件来建立感染。以往的研究表明，树突状细胞在MMTV感染中起一定作用。在这里，我们展示了树突状细胞是体内第一个被MMTV感染的细胞，他们能够产生可以传播给其他细胞类型的传染性病毒。此外，与病毒接触后，树突状细胞变得更成熟，并对趋化因子巨噬细胞炎性蛋白3β做出反应并迁移。最后，我们证明了在体内靶向消融树突状细胞可以显著降低MMTV感染。这些数据表明，MMTV对树突状细胞的感染是病毒在体内最初繁殖的关键。
Mouse mammary tumor virus (MMTV) is a milk-transmitted betaretrovirus that causes mammary tumors in mice. Although mammary epithelial cells are the ultimate targets of MMTV, the virus utilizes components of the host immune system to establish infection. Previous studies indicated that dendritic cells play a role in MMTV infection. Here we show that dendritic cells are the first cells to be infected by MMTV in vivo and that they are capable of producing infectious virus that can be transmitted to other cell types. Moreover, upon contact with the virus, dendritic cells became more mature and migrated in response to the chemokine macrophage inflammatory protein 3 beta. Finally, we demonstrate that targeted ablation of dendritic cells in vivo dramatically attenuated MMTV infection. These data indicate that MMTV infection of dendritic cells is critical to initial propagation of the virus in vivo.