Novel solvent-based method for preparation of alginate beads with improved roundness and predictable size
Novel solvent-based method for preparation of alginate beads with improved roundness and predictable size
复制标题
用于制备具有改进的圆度和可预测尺寸的藻酸盐珠的新型溶剂基方法
DOI：
10.1080/02652040400015478
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发表时间：
2004
影响因子：
3.9
通讯作者：
M. Ansorge‐Schumacher
中科院分区：
文献类型：
--
作者：
A. Buthe;W. Hartmeier;M. Ansorge‐Schumacher
由于分析珠的不适当的圆度，测定藻酸盐凝胶珠内的条件或过程的尝试经常受到不准确的影响。因此，开发了一种新的溶剂型海藻酸盐珠的制备方法：将2%(w/v)海藻酸盐的水溶液注入到由正己烷、正丁醇、正丁醇和1%(w/v)CaCl2以及2%(w/v)CaCl2组成的溶剂分层中。用这种方法制得的直径达3.5mgCl2的微珠的圆度比用海藻酸盐溶液滴入CaCl2硬化液所制得的可比微珠的圆度好∼5%。这是由软件支持的珠子大小和形状的定量分析确定的。此外，新的基于溶剂的方法允许高度可重复性地制备具有精确可预测尺寸的藻酸盐微珠。用这种方法得到的最大珠子直径为9 mm。因此，通过溶剂型海藻酸盐珠的制备，现在可以很容易地获得特定分析目的所需类型的珠子。
Attempts to determine conditions or processes within alginate gel beads often suffer from inaccuracies due to an improper roundness of the analysed beads. Therefore, a novel solvent-based method for the preparation of alginate beads with improved shape was developed: An aqueous solution of 2% (w/v) alginate in water was injected into a solvent layering consisting of hexane, n-butanol, n-butanol with 1% (w/v) CaCl2 and finally 2% (w/v) CaCl2 in water. Beads of up to 3.5 mm in diameter obtained with this method had a roundness which was ∼5% better than comparable beads prepared by dropping an alginate solution into a CaCl2-hardening bath. This was determined by a software supported quantitative analysis of bead size and shape. Additionally, the novel solvent-based method allows for highly reproducible preparation of alginate beads with exactly predictable sizes. The biggest beads obtained with this method were 9 mm in diameter. Thus, with the solvent-based preparation of alginate beads it is now possible to easily obtain beads of exactly the type needed for a specific analytical purpose.