Live imaging of yeast Golgi cisternal maturation
Live imaging of yeast Golgi cisternal maturation
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DOI：
10.1038/nature04737
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发表时间：
2006-06-22
期刊：
影响因子：
64.8
通讯作者：
Nakano, Akihiko
中科院分区：
文献类型：
--
作者：
Matsuura-Tokita, Kumi;Takeuchi, Masaki;Nakano, Akihiko
关于蛋白质运输是如何通过高尔基体进行的有一个争论（1-4）。在分泌途径中，在内质网中合成的分泌蛋白质进入高尔基体的称为顺式池的早期隔室，经历各种修饰和加工，然后从晚期（反式）池离开质膜。货物蛋白必须以顺反方向穿过高尔基体。两个典型的模型提出了囊泡运输或脑池的进展和成熟这一过程。囊泡运输模型预测，高尔基体池是不同的稳定车厢连接的囊泡交通，而池成熟模型预测，池是短暂的结构，从头形成，成熟从顺式到反式，然后消散。人们一直期待着活细胞成像技术的进步来解决这个问题。在这里，我们表明，通过使用高速三维共聚焦显微镜，酵母高尔基体池不改变分布的常驻膜蛋白的顺式性质的反式随着时间的推移，所提出的成熟模型，在一个非常动态的方式。
There is a debate over how protein trafficking is performed through the Golgi apparatus(1-4). In the secretory pathway, secretory proteins that are synthesized in the endoplasmic reticulum enter the early compartment of the Golgi apparatus called cis cisternae, undergo various modifications and processing, and then leave for the plasma membrane from the late ( trans) cisternae. The cargo proteins must traverse the Golgi apparatus in the cis-totrans direction. Two typical models propose either vesicular transport or cisternal progression and maturation for this process. The vesicular transport model predicts that Golgi cisternae are distinct stable compartments connected by vesicular traffic, whereas the cisternal maturation model predicts that cisternae are transient structures that form de novo, mature from cis to trans, and then dissipate. Technical progress in live-cell imaging has long been awaited to address this problem. Here we show, by the use of high-speed three-dimensional confocal microscopy, that yeast Golgi cisternae do change the distribution of resident membrane proteins from the cis nature to the trans over time, as proposed by the maturation model, in a very dynamic way.