How an actin network might cause fountain streaming and nuclear migration in the syncytial Drosophila embryo.
How an actin network might cause fountain streaming and nuclear migration in the syncytial Drosophila embryo.
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DOI：
10.1083/jcb.127.6.1637
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发表时间：
1994-12
期刊：
影响因子：
--
通讯作者：
Schubiger G
中科院分区：
文献类型：
--
作者：
von Dassow G;Schubiger G
在这里，我们使用延时录像带展示了细胞质流动导致果蝇早期合胞胚胎中的核沿前后轴(轴向扩张)迁移。利用共聚焦显微镜和标记的鬼臼蛋白，我们研究了F-肌动蛋白在轴向扩张过程中的分布。我们发现，F-肌动蛋白纤维网络充满了胚胎的细胞质。在轴向扩张过程中，肌动蛋白网络在细胞核周围部分解体。我们对正常发育、固定胚胎和药物注射实验的观察表明，肌动蛋白网络的分解会产生细胞质运动。我们认为，细胞周期调节肌动蛋白网络的分解，这一过程可能直接或间接地由微管介导。我们在轴向扩张过程中观察到的细胞质运动非常类似于阿米巴伪足中的喷泉流动，通过与伪足的类比，我们提出了一个基于肌动蛋白网络内的溶解-收缩耦合的轴向扩张的工作假说。
We show here using time-lapse video tapes that cytoplasmic streaming causes nuclear migration along the anterior-posterior axis (axial expansion) in the early syncytial embryo of Drosophila melanogaster. Using confocal microscopy and labeled phalloidin we explore the distribution of F-actin during axial expansion. We find that a network of F-actin fibers fills the cytoplasm in the embryo. This actin network partially disassembles around the nuclei during axial expansion. Our observations of normal development, fixed embryos, and drug injection experiments indicate that disassembly of the actin network generates cytoplasmic movements. We suggest that the cell cycle regulates disassembly of the actin network, and that this process may be mediated directly or indirectly by the microtubules. The cytoplasmic movements we observe during axial expansion are very similar to fountain streaming in the pseudopod of amoebae, and by analogy with the pseudopod we propose a working hypothesis for axial expansion based on solation-contraction coupling within the actin network.