Live imaging of dorsal root regeneration and the resurgence of a forgotten idea.
Live imaging of dorsal root regeneration and the resurgence of a forgotten idea.
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DOI：
10.1227/01.neu.0000400019.16401.c3
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发表时间：
2011-08
期刊：
影响因子：
4.8
通讯作者：
Amgad S. Hanna;Y. Son;R. Dempsey
中科院分区：
文献类型：
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作者：
Amgad S. Hanna;Y. Son;R. Dempsey
在论文（DiMaio A, Skuba A, Himes BT, et al. Invivoimaging of dorsal rootgenesis: rapid immobilization and presynaptic Differentiation at the CNS/PNS border. J Neurosci. 2011;31(12): 4569-4582）中，作者首次提出了背根（DR）再生的体内成像。动态细胞过程，包括轴突再生以及决定再生成功或失败的轴突与环境之间的相互作用，最好采用通过对每只活体动物进行多次观察来捕获实时事件的技术来研究。由于光学和小鼠转基因方面的革命性创新，我们在体内连续监测神经元的能力显着增强。对活脊髓神经元的成像研究最近才开始，并为静态分析无法解决的生理机制提供了新的见解。作者证明了生长速率为 0.1.5 毫米/天（图 1），与传统已知的 1 毫米/天相反。当到达背根进入区（DREZ）的中枢神经系统（CNS）时，出乎意料的是，这些轴突迅速停滞，然后保持完全不动，即使在调节损伤以增强内在生长潜力之后也是如此。使用来自转基因品系 thy1YFPH 和 thy1-YFP16 的成年小鼠。这些在下表达黄色荧光蛋白（YFP）
I n the paper (DiMaio A, Skuba A, Himes BT, et al. In vivo imaging of dorsal root regeneration: rapid immobilization and presynaptic differentiation at the CNS/PNS border. J Neurosci. 2011;31(12): 4569-4582), the authors present the first in vivo imaging of dorsal root (DR) regeneration. Dynamic cellular processes, including axon regeneration and the interactions between axons and the environment that determine the success or failure of regeneration, are best studied with techniques that capture real-time events with multiple observations of each living animal. Our ability to monitor neurons serially in vivo has increased dramatically owing to revolutionary innovations in optics and mouse transgenics. Imaging studies of neurons in living spinal cord have only recently begun and provided novel insights into physiological mechanisms that static analyses could not have resolved. The authors demonstrated a growth rate of .1.5 mm/d (Figure 1), contrary to the conventionally known 1 mm/d. Upon reaching the central nervous system (CNS) at the dorsal root entry zone (DREZ), unexpectedly, these axons rapidly stalled and then remained completely immobile, even after conditioning lesions that enhanced intrinsic growth potential. Adult mice from transgenic strains thy1YFPH and thy1-YFP16 were used. These express yellow fluorescent protein (YFP) under